泮托拉唑在市售剂型中的动力学分光光度分析。

Kinetic spectrophotometric analysis of pantoprazole in commercial dosage forms.

作者信息

Rahman Nafisur, Bano Zehra, Azmi Syed Najmul Hejaz

机构信息

Department of Chemistry, Aligarh Muslim University, Aligarh 202002, Uttar Predesh, India.

出版信息

Anal Sci. 2006 Jul;22(7):983-8. doi: 10.2116/analsci.22.983.

DOI:10.2116/analsci.22.983

PMID:16837750

Abstract

A kinetic spectrophotometric method has been developed which is based on the oxidation of pantoprazole with Fe(III) in sulfuric acid medium. Fe(III) subsequently reduces to Fe(II), which is coupled with potassium ferricyanide to form Prussian blue. The reaction is followed spectrophotometrically by measuring the increase in absorbance with time (1-8 min) at 725 nm. The initial rate method is adopted for constructing the calibration graph, which is linear in the concentration range of 5-90 microg ml(-1). The regression analysis yields the calibration equation, nu = 3.467 x 10(-6) + 4.356 x 10(-5)C. The limits of detection and quantitation are 1.46 and 4.43 microg ml(-1), respectively. The proposed method was optimized and validated both statistically and through recovery studies. The experimental true bias of all samples is < +/-2.0%. The method has been successfully applied to the determination of pantoprazole in pharmaceutical preparations.

摘要

已开发出一种动力学分光光度法，该方法基于在硫酸介质中泮托拉唑与铁（III）的氧化反应。铁（III）随后还原为铁（II），铁（II）与铁氰化钾反应形成普鲁士蓝。通过在725 nm处测量吸光度随时间（1 - 8分钟）的增加来进行分光光度法跟踪反应。采用初始速率法绘制校准曲线，该校准曲线在5 - 90 μg ml⁻¹的浓度范围内呈线性。回归分析得出校准方程，ν = 3.467×10⁻⁶ + 4.356×10⁻⁵C。检测限和定量限分别为1.46和4.43 μg ml⁻¹。所提出的方法经过优化，并通过统计学方法和回收率研究进行了验证。所有样品的实验真实偏差均<±2.0%。该方法已成功应用于药物制剂中泮托拉唑的测定。