Liu Litao, Li Jing, Li Quanmin
College of Chemistry and Environmental Science, Henan Normal University, Xinxiang, Henan 453007, PR China.
Anal Sci. 2010;26(11):1163-7. doi: 10.2116/analsci.26.1163.
In this paper, a novel method has been established to determine ethamsylate using potassium ferricyanide as a spectroscopic probe reagent. It has been demonstrated that Fe(III) is reduced to Fe(II) by ethamsylate, and that the formed Fe(II) reacts with potassium ferricyanide to form soluble prussian blue (KFe(III)[Fe(II)(CN)(6)]). Beer's law is obeyed in the range of 0.16 - 24.00 µg mL(-1) with the molar absorption coefficient of 2.1 × 10(4) L mol(-1) cm(-1). The detection limit (3 σ/k) is 0.11 µg mL(-1). This method has been successfully applied to determine ethamsylate in pharmaceutical and serum samples with satisfactory results, and presented quite satisfactory credibility during method equivalence assessment.
本文建立了一种以铁氰化钾为光谱探针试剂测定酚磺乙胺的新方法。结果表明，酚磺乙胺可将Fe(III)还原为Fe(II)，生成的Fe(II)与铁氰化钾反应生成可溶性普鲁士蓝（KFe(III)[Fe(II)(CN)(6)]）。在0.16 - 24.00 µg mL(-1)范围内符合比尔定律，摩尔吸收系数为2.1 × 10(4) L mol(-1) cm(-1)。检测限（3σ/k）为0.11 µg mL(-1)。该方法已成功应用于药物和血清样品中酚磺乙胺的测定，结果令人满意，且在方法等效性评估中具有相当令人满意的可信度。
