[以铁氰化铁为试剂分光光度法测定吡罗昔康]

[Spectrophotometric determination of piroxicam using ferric ferricyanide as reagent].

作者信息

Mândrescu Mariana, Spac A F, Dorneanu V

机构信息

Institutul Naţional de Cercetare-Dezvoltare pentru Microbiologie şi Imunologie, I. Cantacuzino" filiala Iaşi.

出版信息

Rev Med Chir Soc Med Nat Iasi. 2009 Jan-Mar;113(1):268-73.

PMID:21495329

Abstract

UNLABELLED

For the piroxicam determination (nonsteroidal antiinflammatory drug-NSAID) it was developed a spectrophotometric method, based on the reduction of ferric ferricyanide into ferro-ferricyanide (Prussian Blue), with maximum of absorbance at 760 nm.

MATERIAL AND METHOD

The practical working conditions were established. In the 0.2 divided by 2.0 microg/mL range of piroxicam concentration, were used the 2 mL of ferric ferricyanide 1 mL of 2N hydrocloric acid. To delay the flocculation of Prussian Blue it was to add a 1 mL solution of sodium lauryl sulfate 1%. After 15 minutes read the absorbance at 760 nm. The developed method was validated.

RESULTS

The method showed a good linearity in the range of 0.2 divided by 2.0 microg/mL (the correlation coefficient r = 0.9995). The detection limit (LD) was 0.056 microg/mL and the quantification limit (LQ) was 0.18 microg/mL. There were established the system precision (RSD = 0.25%), the precison (RSD = 1.91%) and the accuracy-recovery in the range 98.21 divided by 104.92% with a mean recovery of 100.91%.

CONCLUSION

The experimental results demonstrated a good sensibility. The specific absorptivity for this method is A(1 cm, 760 nm)(1%) = 4374 much higher than piroxicam in UV (A(1 cm, 330 nm)(1%) = 296)

摘要

未标记

为了测定吡罗昔康（一种非甾体抗炎药 - NSAID），开发了一种分光光度法，该方法基于将铁氰化铁还原为亚铁氰化铁（普鲁士蓝），在760nm处有最大吸光度。

材料与方法

确定了实际工作条件。在吡罗昔康浓度为0.2至2.0μg/mL的范围内，使用2mL铁氰化铁、1mL 2N盐酸。为了延迟普鲁士蓝的絮凝，加入1mL 1%的十二烷基硫酸钠溶液。15分钟后读取760nm处的吸光度。对所开发的方法进行了验证。

结果

该方法在0.2至2.0μg/mL范围内显示出良好的线性（相关系数r = 0.9995）。检测限（LD）为0.056μg/mL，定量限（LQ）为0.18μg/mL。确定了系统精密度（RSD = 0.25%）、精密度（RSD = 1.91%）以及98.21至104.92%范围内的准确度 - 回收率，平均回收率为100.91%。