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Surface mutagenesis of the bovine papillomavirus E1 DNA binding domain reveals residues required for multiple functions related to DNA replication.牛乳头瘤病毒E1 DNA结合结构域的表面诱变揭示了与DNA复制相关的多种功能所需的残基。
J Virol. 2006 Aug;80(15):7491-9. doi: 10.1128/JVI.00435-06.
2
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本文引用的文献

1
Papillomavirus E1 protein binds to and stimulates human topoisomerase I.乳头瘤病毒E1蛋白与人类拓扑异构酶I结合并对其产生刺激作用。
J Virol. 2006 Feb;80(3):1584-7. doi: 10.1128/JVI.80.3.1584-1587.2006.
2
Assembly of a double hexameric helicase.双六聚体解旋酶的组装
Mol Cell. 2005 Nov 11;20(3):377-89. doi: 10.1016/j.molcel.2005.09.020.
3
The X-ray structure of the papillomavirus helicase in complex with its molecular matchmaker E2.乳头瘤病毒解旋酶与其分子配对物E2复合物的X射线结构。
Genes Dev. 2004 Aug 15;18(16):1981-96. doi: 10.1101/gad.1220104. Epub 2004 Aug 2.
4
Recruitment of replication protein A by the papillomavirus E1 protein and modulation by single-stranded DNA.乳头瘤病毒E1蛋白对复制蛋白A的招募及单链DNA的调控作用
J Virol. 2004 Feb;78(4):1605-15. doi: 10.1128/jvi.78.4.1605-1615.2004.
5
Initiation of DNA replication: lessons from viral initiator proteins.DNA复制的起始：来自病毒起始蛋白的经验教训。
Nat Rev Mol Cell Biol. 2003 Oct;4(10):777-85. doi: 10.1038/nrm1226.
6
Characterization of the minimal DNA binding domain of the human papillomavirus e1 helicase: fluorescence anisotropy studies and characterization of a dimerization-defective mutant protein.人乳头瘤病毒E1解旋酶最小DNA结合结构域的表征：荧光各向异性研究及二聚化缺陷突变蛋白的表征
J Virol. 2003 May;77(9):5178-91. doi: 10.1128/jvi.77.9.5178-5191.2003.
7
Sequential and ordered assembly of E1 initiator complexes on the papillomavirus origin of DNA replication generates progressive structural changes related to melting.乳头瘤病毒DNA复制起点上E1起始复合物的顺序和有序组装产生了与解链相关的渐进性结构变化。
Mol Cell Biol. 2002 Nov;22(21):7712-20. doi: 10.1128/MCB.22.21.7712-7720.2002.
8
Chaperone proteins abrogate inhibition of the human papillomavirus (HPV) E1 replicative helicase by the HPV E2 protein.伴侣蛋白消除了人乳头瘤病毒（HPV）E2蛋白对HPV E1复制解旋酶的抑制作用。
Mol Cell Biol. 2002 Sep;22(18):6592-604. doi: 10.1128/MCB.22.18.6592-6604.2002.
9
Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex.乳头瘤病毒复制起始复合物组装过程中两个中间体的晶体结构。
EMBO J. 2002 Mar 15;21(6):1487-96. doi: 10.1093/emboj/21.6.1487.
10
The E1 initiator recognizes multiple overlapping sites in the papillomavirus origin of DNA replication.E1引发剂识别乳头瘤病毒DNA复制起点中的多个重叠位点。
J Virol. 2001 Jan;75(1):292-302. doi: 10.1128/JVI.75.1.292-302.2001.

牛乳头瘤病毒E1 DNA结合结构域的表面诱变揭示了与DNA复制相关的多种功能所需的残基。

Surface mutagenesis of the bovine papillomavirus E1 DNA binding domain reveals residues required for multiple functions related to DNA replication.

作者信息

Schuck Stephen, Stenlund Arne

机构信息

Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.

出版信息

J Virol. 2006 Aug;80(15):7491-9. doi: 10.1128/JVI.00435-06.

DOI:10.1128/JVI.00435-06
PMID:16840329
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1563737/
Abstract

The E1 protein from papillomaviruses is a multifunctional protein with complex functions required for the initiation of viral DNA replication. We have performed a surface mutagenesis of the well-characterized E1 DNA binding domain (DBD). We demonstrate that substitutions of multiple residues on the surface of the E1 DBD are defective for DNA replication without affecting the DNA binding activity of the protein. The defects of individual substitutions include failure to form the double trimer that melts the ori and failure to form the double hexamer that unwinds the ori. These results demonstrate that the DBD plays an essential role in multiple DNA replication-related processes apart from DNA binding.

摘要

乳头瘤病毒的E1蛋白是一种多功能蛋白，在病毒DNA复制起始过程中具有复杂功能。我们对已充分表征的E1 DNA结合结构域（DBD）进行了表面诱变。我们证明，E1 DBD表面多个残基的取代对DNA复制有缺陷，但不影响该蛋白的DNA结合活性。单个取代的缺陷包括无法形成解开起始位点的双三聚体以及无法形成解开起始位点的双六聚体。这些结果表明，DBD除了DNA结合外，在多个与DNA复制相关的过程中也起着至关重要的作用。