腺相关病毒和腺病毒血清型之间共享的一种依赖胞质动力蛋白的微管结合常见机制。
A common mechanism for cytoplasmic dynein-dependent microtubule binding shared among adeno-associated virus and adenovirus serotypes.
作者信息
Kelkar Samir, De Bishnu P, Gao Guangping, Wilson James M, Crystal Ronald G, Leopold Philip L
机构信息
Department of Genetic Medicine, Weill Medical College of Cornell University, 515 E. 71st Street, S-1000, New York, NY 10021, USA.
出版信息
J Virol. 2006 Aug;80(15):7781-5. doi: 10.1128/JVI.00481-06.
Abstract
During infection, adenovirus-associated virus (AAV) undergoes microtubule-dependent retrograde transport as part of a program of vectorial transport of viral genome to the nucleus. A microtubule binding assay was used to evaluate the hypothesis that cytoplasmic dynein mediates AAV interaction with microtubules. Binding of AAV serotype 2 (AAV2) was enhanced in a nucleotide-dependent manner by the presence of total cellular microtubule-associated proteins (MAPs) but not cytoplasmic dynein-depleted MAPs. Excess AAV2 capsid protein prevented microtubule binding by AAV serotypes 2, 5, and rh.10, as well as adenovirus serotype 5, indicating that similar binding sites are used by these viruses.
摘要
在感染过程中，腺相关病毒（AAV）会经历微管依赖性逆行运输，这是病毒基因组向细胞核进行矢量运输程序的一部分。采用微管结合试验来评估胞质动力蛋白介导AAV与微管相互作用的假说。细胞总微管相关蛋白（MAPs）的存在以核苷酸依赖性方式增强了2型腺相关病毒（AAV2）的结合，但耗尽胞质动力蛋白的MAPs则无此作用。过量的AAV2衣壳蛋白可阻止2型、5型和rh.10型腺相关病毒以及5型腺病毒与微管的结合，这表明这些病毒使用相似的结合位点。
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