Hwang In Koo, Yoo Ki-Yeon, Kim Dae Won, Choi Soo Young, Kang Tae-Cheon, Kim Yong-Sun, Won Moo Ho
Department of Anatomy, College of Medicine, Hallym University, Chunchon 200-702, South Korea.
Neurochem Res. 2006 Jul;31(7):957-65. doi: 10.1007/s11064-006-9101-3. Epub 2006 Jul 14.
Ionized calcium-binding adapter molecule 1 (iba-1) is specifically expressed in microglia and plays an important role in the regulation of the function of microglia. We observed chronological changes of iba-1-immunoreactive cells and iba-1 level in the gerbil hippocampal CA1 region after transient ischemia. Transient forebrain ischemia in gerbils was induced by the occlusion of bilateral common carotid arteries for 5 min. Immunohistochemical and Western blot analysis of iba-1 were performed in the gerbil ischemic hippocampus. In the sham-operated group, iba-1-immunoreactive cells were detected in the CA1 region. Thirty minutes after ischemia/reperfusion, iba-1 immunoreactivity significantly increased, and its immunoreactive cells were well ramified. Three hours after ischemia/reperfusion, iba-1 immunoreactivity and level decreased, and thereafter they increased again with time after ischemia/reperfusion. Three days after ischemia/reperfusion, iba-1-immunoreactive cells had well-ramified processes, which projected to the stratum pyramidale of the CA1 region. Seven days after ischemia/reperfusion, iba-1 immunoreactivity and level were highest in the CA1 region, whereas they significantly decreased in the CA1 region 10 days after ischemia/reperfusion. Iba-1-immunoreactive cells in the ischemic CA1 region were co-localized with OX-42, a microglia marker. In brief, iba-1-immunoreactive cells change morphologically and iba-1 immunoreactivity alters in the CA1 region with time after ischemia/reperfusion. These may be associated with the delayed neuronal death of CA1 pyramidal cells in the gerbil ischemic hippocampus.
离子钙结合衔接分子1（iba-1）在小胶质细胞中特异性表达，在小胶质细胞功能调节中起重要作用。我们观察了沙土鼠短暂性脑缺血后海马CA1区iba-1免疫反应性细胞和iba-1水平的时间变化。通过阻断双侧颈总动脉5分钟诱导沙土鼠短暂性前脑缺血。对沙土鼠缺血海马进行iba-1的免疫组织化学和蛋白质印迹分析。在假手术组中，在CA1区检测到iba-1免疫反应性细胞。缺血/再灌注30分钟后，iba-1免疫反应性显著增加，其免疫反应性细胞分支良好。缺血/再灌注3小时后，iba-1免疫反应性和水平下降，此后随着缺血/再灌注时间的推移再次升高。缺血/再灌注3天后，iba-1免疫反应性细胞具有良好的分支突起，伸向CA1区的锥体层。缺血/再灌注7天后，CA1区iba-1免疫反应性和水平最高，而缺血/再灌注10天后CA1区显著下降。缺血CA1区的iba-1免疫反应性细胞与小胶质细胞标志物OX-42共定位。简而言之，缺血/再灌注后，CA1区iba-1免疫反应性细胞形态发生变化，iba-1免疫反应性随时间改变。这些可能与沙土鼠缺血海马中CA1锥体神经元的延迟性死亡有关。
