Casati Simona, Sager Heinz, Gern Lise, Piffaretti Jean-Claude
Istituto Cantonale di Microbiologia, Bellinzona, Switzerland.
Ann Agric Environ Med. 2006;13(1):65-70.
We have designed and performed a new PCR method based on the 18S rRNA in order to individuate the presence and the identity of Babesia parasites. Out of 1159 Ixodes ricinus (Acari: Ixodidae) ticks collected in four areas of Switzerland, nine were found to contain Babesia DNA. Sequencing of the short amplicon obtained (411-452 bp) allowed the identification of three human pathogenic species: Babesia microti, B. divergens, for the first time in Switzerland, Babesia sp. EU1. We also report coinfections with B. sp. EU1-Borrelia burgdorferi sensu stricto and Babesia sp. EU1-B. afzelii.
我们设计并实施了一种基于18S rRNA的新型聚合酶链反应(PCR)方法,以确定巴贝斯虫寄生虫的存在及其种类。在瑞士四个地区采集的1159只蓖麻硬蜱(蜱螨亚纲:硬蜱科)中,有9只被发现含有巴贝斯虫DNA。对获得的短扩增子(411 - 452 bp)进行测序,首次在瑞士鉴定出三种人类致病物种:微小巴贝斯虫、分歧巴贝斯虫、巴贝斯虫属EU1种。我们还报告了巴贝斯虫属EU1种与狭义伯氏疏螺旋体以及巴贝斯虫属EU1种与阿氏疏螺旋体的混合感染情况。