Ito J, Suzuki J, Kobayashi S
Jpn J Microbiol. 1975 Apr;19(2):87-95. doi: 10.1111/j.1348-0421.1975.tb00854.x.
Pretreatment of mouse L cells with interferon (IF) enhanced IF production in response to polyinosinic-polycytidylic acid (poly I-poly C). Post-treatment of cells with IF caused no significant enhancement of IF production. The enhancing effect of IF pretreatment (priming) reached a maximum after incubation with IF (10 or 100 units/ml) for 4-6 hr at 37 C, but this effect was absent when the incubation was done at 4 C. Cells which were incubated for additional several hours at 37 C after IF pretreatment at 4 C did not develop the primed state nor the antiviral state. The presence of protein synthesis inhibitors during the IF pretreatment depressed, though not completely, the development of the primed state. The residual priming effect was lost when the cells were incubated with the inhibitors at 37 C for 2 hr before they were exposed to poly I-poly C. There was no significant difference in the binding rate of poly I-poly C to cells between IF-treated and untreated cells. The degradation rate of cell-bound poly I-poly C and its sensitivity to exogenous pancreatic ribonuclease in the pretreated cells were also similar to those in the untreated cells.
用干扰素（IF）预处理小鼠L细胞，可增强其对聚肌苷酸-聚胞苷酸（聚I-聚C）的IF产生。用IF对细胞进行后处理不会显著增强IF产生。IF预处理（致敏）的增强作用在37℃下与IF（10或100单位/毫升）孵育4至6小时后达到最大值，但在4℃下孵育时则没有这种作用。在4℃下进行IF预处理后于37℃再孵育数小时的细胞，既未形成致敏状态也未形成抗病毒状态。IF预处理期间存在蛋白质合成抑制剂时，虽未完全抑制，但会抑制致敏状态的形成。当细胞在暴露于聚I-聚C之前于37℃与抑制剂孵育2小时，残余的致敏作用就会消失。IF处理组和未处理组细胞之间，聚I-聚C与细胞的结合率没有显著差异。预处理细胞中细胞结合的聚I-聚C的降解率及其对外源胰核糖核酸酶的敏感性也与未处理细胞中的相似。
