Study of the priming effect of interferon in L cells. I. The primed interferon response and the kinetics of development of priming.

Interferon was detected one hour earlier, its production followed an enhanced pattern and became resistant to actinomycin D 30 minutes sooner in cultures primed by interferon pretreatment before stimulation by polyriboinosinic-polyribocytidylic acid than in unprimed cultures. The kinetics of development of the primed state was found to be a time and dose dependent phenomenon. The continuous presence of interferon during the pretreatment period was not required for the development of the primed state.