中国平胸龟（Platysternon megacephalum）的完整线粒体基因组，其脊椎动物线粒体DNA具有新的基因组织。

Complete mitochondrial genome of Chinese big-headed turtle, Platysternon megacephalum, with a novel gene organization in vertebrate mtDNA.

作者信息

Peng Qiao-Ling, Nie Liu-Wang, Pu You-Guang

机构信息

College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, PR China.

出版信息

Gene. 2006 Sep 15;380(1):14-20. doi: 10.1016/j.gene.2006.04.001. Epub 2006 Apr 28.

DOI:10.1016/j.gene.2006.04.001

PMID:16842936

Abstract

The mitochondrial genome of the Chinese big-headed turtle, Platysternon megacephalum, was obtained using polymerase chain reaction (PCR). The entire mtDNA sequence, the longest mitochondrial genome in turtles reported so far, is 19161 bp. This mitochondrial genome exhibits a novel gene order, which greatly differs from that of any other vertebrates. It is characterized by four distinctive features: 1) the translocation of a gene cluster including three tRNA genes (tRNAHis, tRNASer, tRNALeu(CUN)) and ND5 gene, 2) two tRNAThr pseudogenes, 3) a duplication of pseudo tRNAThr/tRNAPro/D-loop region and 4) 3 non-coding spacers. These unique identities represent a new mitogenomic gene order in vertebrates. The TDRL model was proposed to account for the generation of the gene order in P. megacephalum.

摘要

利用聚合酶链反应（PCR）获得了中国大头龟（Platysternon megacephalum）的线粒体基因组。整个线粒体DNA序列全长19161 bp，是迄今为止报道的龟类中最长的线粒体基因组。该线粒体基因组呈现出一种新颖的基因排列顺序，与其他任何脊椎动物的基因排列顺序都有很大差异。它具有四个显著特征：1）一个包含三个tRNA基因（tRNAHis、tRNASer、tRNALeu(CUN)）和ND5基因的基因簇发生了易位；2）两个tRNAThr假基因；3）假tRNAThr/tRNAPro/D环区域的重复；4）三个非编码间隔区。这些独特特征代表了脊椎动物中一种新的线粒体基因组基因排列顺序。提出了TDRL模型来解释大头龟基因排列顺序的产生。