Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.
Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu, China.
PLoS One. 2022 Jun 2;17(6):e0268064. doi: 10.1371/journal.pone.0268064. eCollection 2022.
The complete mitochondrial genome of Episymploce splendens, 15,802 bp in length, was determined and annotated in this study. The mito-genome included 13 PCGs, 20 tRNAs and 2 rRNAs. Unlike most typical mito-genomes with conservative gene arrangement and exceptional economic organization, E. splendens mito-genome has two tRNAs (tRNA-Gln and tRNA-Met) absence and a long intergenic spacer sequence (93 bp) between tRNA-Val and srRNA, showing the diversified features of insect mito-genomes. This is the first report of the tRNAs deletion in blattarian mito-genomes and we supported the duplication/random loss model as the origin mechanism of the long intergenic spacer. Two Numts, Numt-1 (557 bp) and Numt-2 (975 bp) transferred to the nucleus at about 14.15 Ma to 22.34 Ma, and 19.19 Ma to 24.06 Ma respectively, were found in E. splendens. They can be used as molecular fossils in insect phylogenetic relationship inference. Our study provided useful data for further studies on the evolution of insect mito-genome.
本研究测定并注释了 Episymploce splendens 的完整线粒体基因组，长度为 15802bp。线粒体基因组包括 13 个 PCGs、20 个 tRNAs 和 2 个 rRNAs。与大多数具有保守基因排列和特殊经济组织的典型线粒体基因组不同，E. splendens 线粒体基因组缺失了两个 tRNAs（tRNA-Gln 和 tRNA-Met）和 tRNA-Val 与 srRNA 之间的长基因间隔序列（93bp），表现出昆虫线粒体基因组的多样化特征。这是首次报道直翅目线粒体基因组中的 tRNA 缺失，我们支持重复/随机丢失模型作为长基因间隔起源的机制。在 E. splendens 中发现了两个 Numts，Numt-1（557bp）和 Numt-2（975bp），它们分别在大约 14.15 Ma 到 22.34 Ma 和 19.19 Ma 到 24.06 Ma 时转移到细胞核中。它们可以作为昆虫系统发育关系推断中的分子化石。我们的研究为进一步研究昆虫线粒体基因组的进化提供了有用的数据。
