Zhou Yan, Zhang Jia-Yong, Zheng Rong-Quan, Yu Bao-Gen, Yang Guang
Institute of Ecology, Zhejiang Normal University, Zhejiang, China.
Gene. 2009 Nov 15;447(2):86-96. doi: 10.1016/j.gene.2009.07.009. Epub 2009 Jul 22.
The mt genome of Paa spinosa (Anura: Ranoidae) is a circular molecule of 18,012 bp in length, containing 38 genes (including an extra copy of tRNA-Met gene). This mt genome is characterized by three distinctive features: a cluster of rearranged tRNA genes (LTPF tRNA gene cluster), a tandem duplication of tRNA-Met gene (Met1 and Met2), and distinct repeat regions at both 5' and 3'-sides in the control region. Comparing the locations and the sequences of all tRNA-Met genes among Ranoidae, and constructing NJ tree of the nucleotide of those tRNA-Met genes, we suggested a tandem duplication of tRNA-Met gene can be regarded as a synapomorphy of Dicroglossinae. To further investigate the phylogenetic relationships of anurans, phylogenetic analyses (BI, ML and MP) based on the nucleotide dataset and the corresponding amino acid dataset of 11 protein-coding genes (except ND5 and ATP8) arrived at the similar topology.
棘胸蛙（无尾目：蛙科）的线粒体基因组是一个长度为18,012 bp的环状分子，包含38个基因（包括一个额外的tRNA-Met基因拷贝）。该线粒体基因组具有三个显著特征：一组重排的tRNA基因（LTPF tRNA基因簇）、tRNA-Met基因的串联重复（Met1和Met2）以及控制区5'和3'端的独特重复区域。通过比较蛙科中所有tRNA-Met基因的位置和序列，并构建这些tRNA-Met基因核苷酸的NJ树，我们认为tRNA-Met基因的串联重复可被视为叉舌蛙亚科的共有衍征。为了进一步研究无尾目的系统发育关系，基于11个蛋白质编码基因（除ND5和ATP8外）的核苷酸数据集和相应的氨基酸数据集进行的系统发育分析（贝叶斯推断、最大似然法和最大简约法）得出了相似的拓扑结构。
