Ait El Kadi M, Aghrouch M, Seffar M, El harti J, Bouklouze A, Cherrah Y, Souly K, Zouhdi M
Service de microbiologie, hôpital des spécialités, CHU Ibn-Sina, Rabat, Maroc.
Med Mal Infect. 2006 Jul;36(7):386-9. doi: 10.1016/j.medmal.2006.05.003. Epub 2006 Jul 13.
Metallo-beta-lactamases (MBL) are enzymes produced by Gram-negative bacilli such as Pseudomonas aeruginosa and Acinetobacter baumannii. These enzymes make these isolates resistant to imipenem.
The aim of this study was to determine the prevalence of this resistance mechanism in Pseudomonas aeruginosa and Acinetobacter baumannii strains identified in the bacteriology laboratory of the Rabat Ibn Sina teaching hospital, Morocco.
Screening for MBL was systematic in all resistant strains and/or strains with decreased sensitivity to imipenem, according to Dongeun Yong et al.'s method, using a sterilized solution of EDTA 0.5 M pH 8.
Eighty-five bacterial strains (48 P. aeruginosa and 37 A. baumannii) were identified 23% (11) and 57% (21) of which were respectively resistant to the imipenem. The prevalence of MbetaL producing strains was 27% for P. aeruginosa and 38% for A. baumannii.
These results show that the frequency of these strains is increases in our hospital and that their emergence represents a serious therapeutic and epidemiological problem. This means that we need to implement the supervision of hospital microbial environment and strictly apply hygiene measures.
金属β-内酰胺酶（MBL）是由革兰氏阴性杆菌如铜绿假单胞菌和鲍曼不动杆菌产生的酶。这些酶使这些菌株对亚胺培南耐药。
本研究的目的是确定在摩洛哥拉巴特伊本·西那教学医院细菌学实验室鉴定的铜绿假单胞菌和鲍曼不动杆菌菌株中这种耐药机制的流行情况。
根据Dongeun Yong等人的方法，使用0.5 M pH 8的无菌EDTA溶液，对所有耐药菌株和/或对亚胺培南敏感性降低的菌株进行MBL系统筛查。
共鉴定出85株细菌（48株铜绿假单胞菌和37株鲍曼不动杆菌），其中分别有23%（11株）和57%（21株）对亚胺培南耐药。产MBL菌株的流行率在铜绿假单胞菌中为27%，在鲍曼不动杆菌中为38%。
这些结果表明，我院这些菌株的频率在增加，它们的出现代表了一个严重的治疗和流行病学问题。这意味着我们需要对医院微生物环境实施监测并严格执行卫生措施。
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