使用抗p105单克隆抗体鉴别有丝分裂细胞，以分析依托泊苷和鬼臼毒素在人胃癌细胞中的作用模式。
Discrimination of mitotic cells using anti-p105 monoclonal antibody to analyze the mode of action of etoposide and podophyllotoxin in human gastric cancer cells.
作者信息
Ohyama S, Yonemura Y, Tsugawa K, Miyazaki I, Tanaka M, Sasaki T
机构信息
Department of Surgery II, School of Medicine, Kanazawa University.
出版信息
Jpn J Cancer Res. 1991 Nov;82(11):1258-62. doi: 10.1111/j.1349-7006.1991.tb01790.x.
Anti-p105 monoclonal antibody was used to discriminate between M-phase and G2-phase of gastric cancer cells. p105 is a proliferation-associated nuclear antigen and its expression increases with cell cycle progression, especially in the mitotic phase. As an example of cell cycle analysis, the modes of action of etoposide and podophyllotoxin were examined by multiparameter flow cytometry. We found that etoposide caused G2 block and retarded S phase transit and podophyllotoxin caused potential M phase block in gastric cancer cells. This cell cycle analysis by using anti-p105 monoclonal antibody should be useful for analysis of the actions of anti-tumor agents, especially for M phase analysis, because of its convenience and reliability.
抗p105单克隆抗体用于区分胃癌细胞的M期和G2期。p105是一种与增殖相关的核抗原，其表达随细胞周期进程增加，尤其在有丝分裂期。作为细胞周期分析的一个例子，通过多参数流式细胞术检测了依托泊苷和鬼臼毒素的作用方式。我们发现依托泊苷导致G2期阻滞并延缓S期过渡，而鬼臼毒素在胃癌细胞中导致潜在的M期阻滞。由于其便利性和可靠性，这种使用抗p105单克隆抗体的细胞周期分析对于抗肿瘤药物作用的分析应该是有用的，特别是对于M期分析。
Zotero 插件