Clevenger C V, Epstein A L, Bauer K D
Cytometry. 1987 May;8(3):280-6. doi: 10.1002/cyto.990080308.
The quantification of an interchromatin-associated antigen, designated p 105, during cellular passage through mitosis is described. Indirect immunofluorescence microscopy and immunogold electron microscopy demonstrated a qualitative increase in p 105 within the mitotic cytoplasm. Multiparameter flow cytometric analysis was performed on fixed cells sequentially stained with anti-p 105 immunofluorescence and/or propidium iodide. This analysis demonstrated approximately a tenfold increase in intracellular p 105 content as a function of progression from the G2 to the M phase. This increase was corroborated by the quantitative immunoblot analysis of colchicine-treated cell cultures and of cells sorted on the basis of anti-p 105 immunofluorescence. The data reveal that the increased levels of anti-p 105 immunofluorescence in conjunction with flow cytometry may be used effectively to quantitate mitotic index and isolate mitotic cells. The function and modulation of p 105 throughout the cell cycle is discussed.
本文描述了在细胞有丝分裂过程中，一种名为p105的染色质间相关抗原的定量情况。间接免疫荧光显微镜和免疫金电子显微镜显示，有丝分裂细胞质中的p105在质量上有所增加。对先用抗p105免疫荧光和/或碘化丙啶顺序染色的固定细胞进行多参数流式细胞术分析。该分析表明，随着细胞从G2期进入M期，细胞内p105含量大约增加了10倍。秋水仙碱处理的细胞培养物以及基于抗p105免疫荧光分选的细胞的定量免疫印迹分析证实了这种增加。数据表明，抗p105免疫荧光水平的增加与流式细胞术相结合，可有效地用于定量有丝分裂指数和分离有丝分裂细胞。文中还讨论了p105在整个细胞周期中的功能和调节。
