Filho J C, Bergström J, Stehle P, Fürst P
Division of Renal Medicine, Department of Clinical Science, Huddinge University Hospital, Karolinska Institute, Stockholm, Sweden.
Clin Nutr. 1997 Dec;16(6):299-305. doi: 10.1016/s0261-5614(97)80015-5.
Muscle biopsy and blood samples were simultaneously collected from 27 healthy subjects for determination of free amino acid (AA) in plasma, muscle and erythrocytes (RBC) by reverse-phase high performance liquid chromatography and related their concentrations to deoxyribonucleic acid (DNA), muscle alkali-soluble protein, s-albumin and s-total protein. Extra- and intracellular water distribution in muscle was estimated with a modified chloride method. The majority of the AA showed higher concentration in intracellular water (ICW) than in plasma; exceptions were the concentrations of branched-chain amino acids (BCAA), phenylalanine and tyrosine exhibiting muscle/plasma and RBC/plasma ratios of 1.0-1.4. Muscle arginine and lysine as well as RBC citrulline showed higher concentrations in younger female subjects when compared to males whereas in the three compartments all other AA were present in higher concentrations in the males than in the female subjects. The levels of valine and asparagine in muscle as well as serine, ornithine, methionine and BCAA in RBC were higher in the younger than in the elderly subjects. On the other hand, muscle arginine and plasma histidine levels were higher in the elderly than in the younger subjects. There were significant positive correlations between plasma, muscle and RBC AA concentrations for a large number of AA, specially BCAA. The RBC BCAA concentrations correlated most strongly with the ratio of alkali-soluble protein to DNA in skeletal muscle (P < 0.002) and were also significantly correlated to serum albumin and total proteins. The results presented here are proposed to be used as reference data for simultaneously comparing the AA profile in muscle, plasma and RBC in various disease conditions.
从27名健康受试者身上同时采集肌肉活检样本和血样，采用反相高效液相色谱法测定血浆、肌肉和红细胞中的游离氨基酸（AA），并将其浓度与脱氧核糖核酸（DNA）、肌肉碱溶性蛋白、血清白蛋白和血清总蛋白进行关联。采用改良的氯化物法估算肌肉中的细胞外和细胞内水分分布。大多数氨基酸在细胞内液（ICW）中的浓度高于血浆；例外的是支链氨基酸（BCAA）、苯丙氨酸和酪氨酸的浓度，其肌肉/血浆和红细胞/血浆的比值为1.0 - 1.4。与男性相比，年轻女性受试者的肌肉精氨酸和赖氨酸以及红细胞瓜氨酸浓度更高，而在三个组分中，所有其他氨基酸在男性中的浓度均高于女性受试者。年轻受试者肌肉中的缬氨酸和天冬酰胺以及红细胞中的丝氨酸、鸟氨酸、蛋氨酸和BCAA水平高于老年受试者。另一方面，老年受试者的肌肉精氨酸和血浆组氨酸水平高于年轻受试者。大量氨基酸（特别是BCAA）的血浆、肌肉和红细胞AA浓度之间存在显著正相关。红细胞BCAA浓度与骨骼肌中碱溶性蛋白与DNA的比值相关性最强（P < 0.002），并且与血清白蛋白和总蛋白也显著相关。本文给出的结果建议用作在各种疾病状态下同时比较肌肉、血浆和红细胞中氨基酸谱的参考数据。
