Liang Jing, Bian Mei-Lu, Chen Qing-Yun, Liu Xia, Ou Hua, Li Min, Liu Jun
Department of Obstetrics and Gynecology, China-Japan Friendship Hospital, Beijing 100029.
Chin Med Sci J. 2006 Jun;21(2):81-5.
To evaluate the overexpression of cyclin G1 in cervical intraepithelial neoplasia (CIN) and cervical carcinoma, and the correlation between cyclin G1 and high-risk human papilloma virus (HPV) infection.
All of the specimens were obtained from the Department of Pathology of China-Japan Friendship Hospital from January 2000 to August 2004. We detected the expression of cyclin G1 with immunohistochemistry, HPV16/18 infection with in situ hybridization, and high-risk HPV infection with Hybrid capture system II (HC-II) in normal group (25 cases), CIN I (48 cases), CIN II (56 cases), CIN III (54 cases), and invasive cervical squamous-cell carcinoma (SCC, 31 cases).
The positive rates of cyclin G1 expression in CIN (77.85%) and SCC cervical tissues (87.10%) were significantly higher than normal (8.00%, P < 0.01), and the intensities of cyclin G1 expression in CIN (40.60%) and SCC cervical tissues (61.51%) were significantly higher than normal (2.72%, P < 0.05). The positive rates and intensities of cyclin G1 expression increased gradually with the grade of cervical lesions. High-risk HPV infection rates were higher in CIN and SCC than normal groups (P < 0.05). There was a positive correlation between cyclin G1 expression and high-risk HPV infection detected with HC-II (Kendall's tau-b = 0.316, 0.269, 0.352, and 0.474 in CIN I, CINII, CIN III, and SCC, respectively, P < 0.05).
Cyclin G1 is overexpressed in CIN and SCC. Cyclin G1 may be a biomarker for detecting CIN and SCC. Cyclin G1 may play an important role in the oncogenesis of CIN and SCC by high-risk HPV infection.
评估细胞周期蛋白G1（cyclin G1）在宫颈上皮内瘤变（CIN）和宫颈癌中的过表达情况，以及cyclin G1与高危型人乳头瘤病毒（HPV）感染之间的相关性。
所有标本均取自2000年1月至2004年8月中日友好医院病理科。我们采用免疫组织化学检测cyclin G1的表达，原位杂交检测HPV16/18感染，采用杂交捕获系统II（HC-II）检测正常组（25例）、CIN I（48例）、CIN II（56例）、CIN III（54例）和宫颈浸润性鳞状细胞癌（SCC，31例）中的高危型HPV感染。
CIN（77.85%）和宫颈SCC组织（87.10%）中cyclin G1表达的阳性率显著高于正常组（8.00%，P<0.01），CIN（40.60%）和宫颈SCC组织（61.51%）中cyclin G1表达的强度显著高于正常组（2.72%，P<0.05）。cyclin G1表达的阳性率和强度随宫颈病变级别逐渐升高。CIN和SCC中的高危型HPV感染率高于正常组（P<0.05）。采用HC-II检测发现，cyclin G1表达与高危型HPV感染之间存在正相关（CIN I、CINII、CIN III和SCC中Kendall's tau-b分别为0.316、0.269、0.352和0.474，P<0.05）。
Cyclin G1在CIN和SCC中过表达。Cyclin G1可能是检测CIN和SCC的生物标志物。Cyclin G1可能通过高危型HPV感染在CIN和SCC的肿瘤发生中起重要作用。
