一种用于拟除虫菊酯类杀虫剂氯氟氰菊酯的酶联免疫吸附测定法的开发。

Development of an enzyme-linked immunosorbent assay for the pyrethroid insecticide cyhalothrin.

作者信息

Gao Hongbin, Ling Yun, Xu Ting, Zhu Weiwen, Jing Hongyu, Sheng Wei, Li Qing X, Li Ji

机构信息

Department of Ecology and Environmental Sciences, College of Resource and Environment and College of Natural Sciences, China Agricultural University, Beijing, 100094, People's Republic of China.

出版信息

J Agric Food Chem. 2006 Jul 26;54(15):5284-91. doi: 10.1021/jf0607009.

DOI:10.1021/jf0607009

PMID:16848507

Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) was developed for detection of the pyrethroid insecticide cyhalothrin. Three haptens with an amine or propanoic acid terminus were synthesized and then conjugated with bovine serum albumin to give immunogens. Eight polyclonal antisera produced by rabbits were screened for titers and affinity using three different coating antigens. The antiserum CWB-C had the highest affinity with cyhalothrin and a low affinity with fenvalerate, fenpropathrin, deltamethrin, and fluvalinate. The half-maximum inhibition concentration for cyhalothrin was 37.2 microg/L, and the limit of detection was 4.7 microg/L. The recoveries of different concentrations of cyhalothrin (0.1-2500 microg/L) from fortified tap water, well water, and wastewater samples as determined with the ELISA were 81-114%.

摘要

开发了一种竞争性酶联免疫吸附测定法（ELISA）用于检测拟除虫菊酯类杀虫剂氯氟氰菊酯。合成了三种带有胺基或丙酸末端的半抗原，然后将其与牛血清白蛋白偶联以制备免疫原。使用三种不同的包被抗原来筛选由兔子产生的八种多克隆抗血清的效价和亲和力。抗血清CWB-C与氯氟氰菊酯具有最高的亲和力，而与氰戊菊酯、甲氰菊酯、溴氰菊酯和氟胺氰菊酯的亲和力较低。氯氟氰菊酯的半数抑制浓度为37.2μg/L，检测限为4.7μg/L。用ELISA测定，加标自来水、井水和废水样品中不同浓度氯氟氰菊酯（0.1 - 2500μg/L）的回收率为81 - 114%。