Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China.
J Agric Food Chem. 2011 Apr 13;59(7):2997-3003. doi: 10.1021/jf104914d. Epub 2011 Mar 16.
A sensitive and broad class selective direct competitive enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody (McAb) has been described for the detection of pyrethroids with phenoxybenzene group. One monoclonal antibody, 2G(2)E(7), was obtained and characterized after fusion of myeloma cells with spleen cells isolated from BALB/c mice. The assay with the most selectivity for the family pyrethroids with phenoxybenzene group was optimized. The IC(50) values of the optimized immunoassay were 1.8 μg L(-1) for deltamethrin, 1.5 μg L(-1) for cypermethrin, 2.0 μg L(-1) for fluvalinate and fenvalerate, 2.2 μg L(-1) for phenothrin, 2.4 μg L(-1) for flucythrinate, 3.0 μg L(-1) for fenpropathrin, and 5.0 μg L(-1) for permethrin. River water samples fortified with pyrethroids were analyzed with the ELISA to evaluate the accuracy of the assay. The recoveries of pyrethroids in spiked water samples ranged from 74 to 108%. The results indicate that the ELISA developed can accurately simultaneously determine pyrethroids with phenoxybenzene group in water samples.
一种基于单克隆抗体的、对含苯氧苄基的拟除虫菊酯具有高灵敏度和广谱选择性的直接竞争酶联免疫吸附测定法（ELISA）已被描述。通过融合骨髓瘤细胞和 BALB/c 小鼠的脾细胞，获得并鉴定了一种单克隆抗体 2G(2)E(7)。对具有苯氧苄基的拟除虫菊酯家族进行了最具选择性的测定优化。优化后的免疫测定法的 IC(50)值分别为：溴氰菊酯 1.8μg/L、氯氰菊酯 1.5μg/L、氟丙菊酯和高效氯氟氰菊酯 2.0μg/L、苯醚菊酯 2.2μg/L、氟氯氰菊酯 2.4μg/L、溴氰菊酯 3.0μg/L 和氯菊酯 5.0μg/L。用 ELISA 分析加标河水样品，以评估该测定法的准确性。在加标水样中的拟除虫菊酯回收率在 74%至 108%之间。结果表明，该 ELISA 可准确、同时测定水样中的含苯氧苄基的拟除虫菊酯。
