用于监测环境和人体中拟除虫菊酯类杀虫剂暴露的免疫分析和生物传感器。
Immunoassays and biosensors for monitoring environmental and human exposure to pyrethroid insecticides.
机构信息
Department of Entomology, University of California, Davis, Davis, California 95616, United States.
出版信息
J Agric Food Chem. 2011 Apr 13;59(7):2792-802. doi: 10.1021/jf1033569. Epub 2010 Nov 24.
Abstract
This paper describes some of the early work on pyrethroid insecticides in the Casida laboratory and briefly reviews the development and application of immunochemical approaches for the detection of pyrethroid insecticides and their metabolites for monitoring environmental and human exposure. Multiple technologies can be combined to enhance the sensitivity and speed of immunochemical analysis. The pyrethroid assays are used to illustrate the use of some of these immunoreagents such as antibodies, competitive mimics, and novel binding agents such as phage-displayed peptides. The paper also illustrates reporters such as fluorescent dyes, chemiluminescent compounds, and luminescent lanthanide nanoparticles, as well as the application of magnetic separation, and automatic instrumental systems, biosensors, and novel immunological technologies. These new technologies alone and in combination result in an improved ability to both determine if effective levels of pyrethroids are being used in the field and evaluate possible contamination.
摘要
本文介绍了凯赛实验室在拟除虫菊酯杀虫剂早期的一些工作，并简要回顾了免疫化学方法的发展和应用，这些方法用于检测拟除虫菊酯杀虫剂及其代谢物，以监测环境和人体暴露情况。多种技术可以结合使用，以提高免疫化学分析的灵敏度和速度。本文还以拟除虫菊酯检测为例，说明了一些免疫试剂的应用，如抗体、竞争性模拟物和新型结合剂，如噬菌体展示肽。本文还介绍了报告物，如荧光染料、化学发光化合物和发光镧系纳米粒子，以及磁分离、自动仪器系统、生物传感器和新型免疫技术的应用。这些新技术单独使用和组合使用，都提高了确定田间是否使用有效浓度的拟除虫菊酯以及评估可能污染的能力。
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