特布他林对油酸诱导的大鼠肺损伤后肺泡液体清除率的影响。

Effect of terbutaline on alveolar liquid clearance after oleic acid-induced lung injury in rats.

作者信息

Tao Jun, Yang Tian-de, Li Hong, Du Zhi-yong

机构信息

Department of Anesthesiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.

出版信息

Chin J Traumatol. 2006 Aug;9(4):211-6.

PMID:16848992

Abstract

OBJECTIVE

To investigate whether terbutaline affects alveolar liquid clearance after oleic acid-induced lung injury in rats.

METHODS

Forty healthy Wistar rats (weighing 250-280 g) were randomly divided into five groups (n=8 in each group): the normal control group (control group), oleic acid injury group (injury group), terbutaline-treated group (terbutaline group), terbutaline plus amiloride-treated group (terbutaline+amiloride group) and terbutaline plus ouabain-treated group (terbutaline+ouabain group). Acute lung injury model was induced by intravenous oleic acid (0.25 ml/kg body weight). 24 hours later, 1.5 microCi (125) I-labeled 5% albumin solution (5 ml/kg body weight) was dripped into the lungs through trachea. The alveolar liquid clearance rate, extravascular lung water content, and arterial blood gas were measured 1 hour thereafter.

RESULTS

At 24 hours after infusion of oleic acid, the rats developed pulmonary edema and severe hypoxemia, with the alveolar liquid clearance rate decreased by 49.2% and the extravascular lung water content elevated by 47.9%. Compared with the rats in the injury group, terbutaline (10(-4) mol/L) significantly increased the alveolar liquid clearance rate, decreased the extravascular lung water content and improved hypoxemia. The effect of terbutaline was partly blocked by amiloride and ouabain, which were inhibitors of sodium transport. Terbutaline increased the alveolar liquid clearance rate by 63.7%, and amiloride and ouabain reduced the alveolar liquid clearance rate by 54.7% and 56.8%, respectively.

CONCLUSIONS

Terbutaline can accelerate alveolar liquid clearance through increasing sodium transport to attenuate pulmonary edema, thus improving gas exchange, which may have therapeutical effect on pulmonary edema after acute lung injury.

摘要

目的

研究特布他林对油酸诱导的大鼠肺损伤后肺泡液体清除率的影响。

方法

40只健康Wistar大鼠（体重250 - 280 g）随机分为五组（每组n = 8）：正常对照组（对照组）、油酸损伤组（损伤组）、特布他林治疗组（特布他林组）、特布他林加阿米洛利治疗组（特布他林 + 阿米洛利组）和特布他林加哇巴因治疗组（特布他林 + 哇巴因组）。通过静脉注射油酸（0.25 ml/kg体重）诱导急性肺损伤模型。24小时后，将1.5微居里（125）I标记的5%白蛋白溶液（5 ml/kg体重）经气管滴入肺内。此后1小时测量肺泡液体清除率、血管外肺水含量和动脉血气。

结果

注入油酸24小时后，大鼠出现肺水肿和严重低氧血症，肺泡液体清除率降低49.2%，血管外肺水含量升高47.9%。与损伤组大鼠相比，特布他林（10^(-4) mol/L）显著提高了肺泡液体清除率，降低了血管外肺水含量并改善了低氧血症。特布他林的作用部分被钠转运抑制剂阿米洛利和哇巴因阻断。特布他林使肺泡液体清除率提高了63.7%，阿米洛利和哇巴因分别使肺泡液体清除率降低了54.7%和56.8%。