Sakuma T, Suzuki S, Usuda K, Handa M, Okaniwa G, Nakada T, Fujimura S
Department of Surgery, Sendai Kosei Hospital, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1995 Sep;33(9):966-72.
Little information is available regarding the effect of ion transport agonists and antagonists on ion transport in the human lung. Therefore, we studied ion transport in lungs resected from patients with lung cancer. A test solution of 45 ml of isosmotic albumin was instilled into one segment of a resected lobe within 10 min of resection. Because protein leaves the air space very slowly, the concentration of alveolar protein over 4 h was used to quantify the volume of alveolar fluid. Ion transport was measured from the changes in ion concentrations and the volume of alveolar fluid. In the basal condition, the net efflux of Na+ and Cl- were 4.66 +/- 0.83 mEq/l/h and 3.52 +/- 0.84 mEq/l/h, respectively. In contrast, the net influx of K+ was 0.44 +/- 0.07 mEq/l/h. Amiloride (10(-5) M), an inhibitor of apical Na+ uptake, ouabain (10(-3) M), an inhibitor of Na(+)-K+ ATPase, and hypothermia (8 degrees C) reduced the efflux of Na+ and Cl-. Ouabain and hypothermia increased the net influx of K+. Terbutaline (10(-3) or 10(-4) M) increased the efflux of Na+ and Cl-, but did not affect the influx of K+. Propranolol (10(-4) M) and amiloride (10(-5) M) inhibited the terbutaline-induced increase in the transport of Na+ and Cl-. Alveolar fluid clearance was closely correlated with Na+ transport and with Cl- transport. However, the values of Na+ transport were greater than those of Cl- transport. These data suggest that Na+ transport is accompanied by Cl- transport and fluid movement out of the alveolar space in resected human lungs.
关于离子转运激动剂和拮抗剂对人肺离子转运的影响，目前可用信息较少。因此，我们研究了从肺癌患者切除的肺组织中的离子转运。在切除后10分钟内，将45毫升等渗白蛋白测试溶液滴注到切除肺叶的一个节段中。由于蛋白质离开气腔的速度非常慢，因此用4小时内肺泡蛋白的浓度来量化肺泡液的体积。通过离子浓度和肺泡液体积的变化来测量离子转运。在基础状态下，Na⁺和Cl⁻的净流出量分别为4.66±0.83 mEq/l/h和3.52±0.84 mEq/l/h。相比之下，K⁺的净流入量为0.44±0.07 mEq/l/h。氨氯地平（10⁻⁵ M），一种顶端Na⁺摄取抑制剂；哇巴因（10⁻³ M），一种Na⁺-K⁺ ATP酶抑制剂；以及低温（8℃）降低了Na⁺和Cl⁻的流出。哇巴因和低温增加了K⁺的净流入。特布他林（10⁻³或10⁻⁴ M）增加了Na⁺和Cl⁻的流出，但不影响K⁺的流入。普萘洛尔（10⁻⁴ M）和氨氯地平（10⁻⁵ M）抑制了特布他林诱导的Na⁺和Cl⁻转运增加。肺泡液清除率与Na⁺转运和Cl⁻转运密切相关。然而，Na⁺转运的值大于Cl⁻转运的值。这些数据表明，在切除的人肺中，Na⁺转运伴随着Cl⁻转运以及液体从肺泡腔中流出。
