Azarmi Shirzad, Huang Yuan, Chen Hua, McQuarrie Steve, Abrams Douglas, Roa Wilson, Finlay Warren H, Miller Gerald G, Löbenberg Raimar
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton , Alberta, Canada.
J Pharm Pharm Sci. 2006;9(1):124-32.
To establish a matrix of parameters to synthesize nanoparticles of different sizes and to investigate the cellular uptake of these nanoparticles by osteosarcoma cancer cells in order to investigate their potential as therapeutic drugdelivery carriers.
Gelatin A and B were used to synthesize nanoparticles by a two-step desolvation process. Different parameters were investigated, including temperature, pH, concentration of glutaraldehyde, type of desolvating agent and nature of gelatin. For cell uptake studies, Texas Red labeled nanoparticles were incubated with 143B osteosarcoma cells and then evaluated using confocal laser scanning microscopy (CLSM).
The systematic investigation of the synthesis parameters showed that it is possible to prepare gelatin-based nanoparticles with different particle sizes and a narrow size distribution. Temperature and nature of the gelatin were the most important synthesis factors. Bioimaging using CLSM showed uptake of the nanoparticles by 143B osteosarcoma cancer cells.
Osteosarcoma cancer cells take up gelatin nanoparticles. This might improve the clinical effectiveness of anti-cancer treatments if nanoparticles are used as a drug delivery system and has important implications for future cancer treatment strategies.
建立一个参数矩阵以合成不同尺寸的纳米颗粒，并研究骨肉瘤癌细胞对这些纳米颗粒的细胞摄取情况，从而探究其作为治疗性药物递送载体的潜力。
使用明胶A和B通过两步去溶剂化过程合成纳米颗粒。研究了不同参数，包括温度、pH值、戊二醛浓度、去溶剂化剂类型和明胶性质。对于细胞摄取研究，将Texas Red标记的纳米颗粒与143B骨肉瘤细胞孵育，然后使用共聚焦激光扫描显微镜（CLSM）进行评估。
对合成参数的系统研究表明，可以制备具有不同粒径且粒径分布窄的明胶基纳米颗粒。温度和明胶性质是最重要的合成因素。使用CLSM进行的生物成像显示143B骨肉瘤癌细胞摄取了纳米颗粒。
骨肉瘤癌细胞摄取明胶纳米颗粒。如果将纳米颗粒用作药物递送系统，这可能会提高抗癌治疗的临床效果，并对未来的癌症治疗策略具有重要意义。
