半夏丛生芽诱导与快速繁殖
[Clustered shoot induction and rapid propagation on Pinellia ternate].
作者信息
Xu Xiumei, Lu Daoxi, Sun Min, Zhang Ming, Chen Min
机构信息
School of Life Sciences, Southwest University, Chongqing.
出版信息
Zhong Yao Cai. 2005 Dec;28(12):1052-3.
OBJECT
To establish the rapid propagation system of Pinellia ternate.
METHOD
The Clustered shoots were induced by the explant lamina and petiole from the terminal bud of Pinellia ternate tuber in test tube.
RESULT
The plantlets could regenerate from the lamina and petiole directly without via the callus. The MS Medium with 2.0 mg/L 6-BA and 0.1 mg/L NAA was optimal for the induction of culstered shoots from explants of lamina; the MS with 2.0 mg/L 6-BA and 0.25 mg/L NAA was best for the explants of petiole; and the 1/2 MS with 1.0 mg/L KT and 2.0 mg/L NAA was the better medium for induction of roots.
CONCLUSION
The regenerated rate of Pinellia ternata can improve by inducing the clustered shoots from the explants of lamina and petiole directly.
目的
建立半夏快速繁殖体系。
方法
以半夏块茎顶芽的叶片和叶柄为外植体，在试管中诱导丛生芽。
结果
叶片和叶柄可直接再生出植株，无需经过愈伤组织。对于叶片外植体，添加2.0 mg/L 6 - BA和0.1 mg/L NAA的MS培养基最适合诱导丛生芽；对于叶柄外植体，添加2.0 mg/L 6 - BA和0.25 mg/L NAA的MS培养基最佳；而添加1.0 mg/L KT和2.0 mg/L NAA的1/2 MS培养基对诱导生根效果较好。
结论
通过直接从叶片和叶柄外植体诱导丛生芽，可提高半夏的再生率。
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