[喜树愈伤组织的诱导与培养]

[Callus induction and cultivation of Camptotheca acuminata].

作者信息

Ma Lin

机构信息

School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 Jul;32(13):1273-6.

PMID:17879723

Abstract

OBJECTIVE

To study the callus induction from leaf, stem segments and stem segments with axillary's bud and the subculture conditions of callus in Camptotheca acuminata.

METHOD

The explants were inoculated into the media of MS with different concentrations of 6-BA, NAA and 2, 4-D by orthogonal experiment.

RESULT AND CONCLUSION

The optimal medium for callus induction was MS with 6-BA 2 mg x L(-1), NAA 2 mg x L(-1) and 2,4-D 2 mg x L(-1). It had better effects in leaf than in other explants. The induction ratio in leaf reached above 80%. The callus subcultured on the medium of MS with 6-BA 1 mg x L(-1) and 2, 4-D 1 mg x L(-1) grew vigorous and more quickly than that in other media. It was loose, friable in consistency and suitable for cell culture.

摘要

目的

研究喜树叶片、茎段及带腋芽茎段的愈伤组织诱导以及愈伤组织的继代培养条件。

方法

通过正交试验将外植体接种到添加不同浓度6 - 苄氨基腺嘌呤（6 - BA）、萘乙酸（NAA）和2,4 - 二氯苯氧乙酸（2,4 - D）的MS培养基中。

结果与结论

愈伤组织诱导的最佳培养基为添加6 - BA 2毫克/升、NAA 2毫克/升和2,4 - D 2毫克/升的MS培养基。该培养基对叶片的诱导效果优于其他外植体。叶片的诱导率达到80%以上。在添加6 - BA 1毫克/升和2,4 - D 1毫克/升的MS培养基上继代培养的愈伤组织生长旺盛且比在其他培养基上生长更快。其质地疏松、易碎，适合细胞培养。