Röth E, Török B
Department of Experimental Surgery, University Medical School, Pécs, Hungary.
Basic Res Cardiol. 1991 Sep-Oct;86(5):422-33. doi: 10.1007/BF02190710.
The left descending coronary artery (LAD) was ligated for 45 min or 90 min followed by 1-h reperfusion. During experiments the animals in Group I (15 dogs) received saline infusion, in Group II (20 dogs) they received the ultrashort-acting beta-blocker Brevibloc (esmolol HCl). The marker of lipid peroxidation the malondialdehyde (MDH) as well as endogen scavengers, the glutathione (GSH), and superoxide dismutase (SOD) were measured in the heart tissue homogenates. In blood and heart tissue samples the 6-keto-prostaglandin F1 alpha (PGF1 alpha) and thromboxane B2 (TXB2) were determined. Biochemical measurements revealed that esmolol HCl has beneficial effect on the free-radical-meduated-damage reducing the MDA content in the ischemic area. In Group I the value of MDA after 90 min of LAD ligature was 138 +/- 5.6%, in Group II the elevation was only 107.4 +/- 3.2%. After treatment with Brevibloc the GSH content of ischemic-reperfused areas decreased slightly (81.75 +/- 3.5% of the normal value), moreover, in Group I the depletion of GSH was considerable (64.5 +/- 4.2%). Coronary reperfusion caused the release of eicosanoids in both groups, mainly in the first 10 min. The highest value of thromboxane in blood samples could be measured in Group I after 90 min of LAD ligature (24.8 +/- 3.6 pmol/ml; the normal value 8-12 pmol/ml). In Group II during the same period of experiments the TXB2 in the blood was 14 +/- 3.7 pmol/ml. In heart tissue samples the amount of endoperoxides increased in ischemic and non-ischemic areas of Group I and II. Nevertheless, the calculated ratio of PGF1 alpha and TXB2 was near to the normal after Brevibloc treatment (0.85-0.9; the normal values were 1-1.2). These results indicate that esmolol HCl can modulate both the free-radical-mediated reaction and arachidonic acid metabolism.
结扎左冠状动脉前降支（LAD）45分钟或90分钟，随后再灌注1小时。实验期间，第一组（15只犬）动物输注生理盐水，第二组（20只犬）动物接受超短效β受体阻滞剂Brevibloc（盐酸艾司洛尔）。在心脏组织匀浆中测量脂质过氧化标志物丙二醛（MDH）以及内源性清除剂谷胱甘肽（GSH）和超氧化物歧化酶（SOD）。在血液和心脏组织样本中测定6-酮-前列腺素F1α（PGF1α）和血栓素B2（TXB2）。生化测量显示，盐酸艾司洛尔对自由基介导的损伤具有有益作用，可降低缺血区域的丙二醛含量。在第一组中，LAD结扎90分钟后丙二醛值为138±5.6%，在第二组中，升高仅为107.4±3.2%。用Brevibloc治疗后，缺血再灌注区域的GSH含量略有下降（为正常值的81.75±3.5%），此外，在第一组中GSH的消耗相当大（64.5±4.2%）。两组冠状动脉再灌注均导致类花生酸释放，主要在最初10分钟内。LAD结扎90分钟后，第一组血液样本中血栓素的最高值为24.8±3.6 pmol/ml（正常值为8 - 12 pmol/ml）。在第二组实验的同一时期，血液中的TXB2为14±3.7 pmol/ml。在第一组和第二组的缺血和非缺血区域，心脏组织样本中的内过氧化物量均增加。然而，用Brevibloc治疗后，计算得出的PGF1α与TXB2的比值接近正常（0.85 - 0.9；正常值为1 - 1.2）。这些结果表明，盐酸艾司洛尔可调节自由基介导的反应和花生四烯酸代谢。
