[Effect of cardiac sympathetic nerves on changes of TXB2 and 6-keto-PGF1 alpha in venous blood of ischemic myocardium].

Experiments were performed on 54 anesthetized, open-chest dogs to observe the effect of cardiac sympathetic nerves and alpha- and beta-adrenoceptor blockades on changes of platelet function during the early phase of myocardial ischemia. It was shown that contents of TXB2 and 6-keto-PGF1 alpha after coronary occlusion were markedly increased, and the platelet count was reduced in the coronary venous blood of ischemic myocardium. It was found that the changes in parameters of platelet function after coronary occlusion were significantly relieved by topical application of lignocaine-soaked gauze pads to the ischemic area of the heart or by bilateral stellectomy for blocking impulse of afferent and efferent of cardiac sympathetic nerves, compared with changes in parameters of control group, P less than 0.01. On the other hand, changes in parameters of the control group could be restored by intravenous infusion of noradrenaline to dogs which bilateral stellectomy was performed on, but not by infusion of normal saline. Different effects of alpha- and beta-adrenoceptor blockades on the changes in aforementioned parameters were found. The changing degrees in elevation of TXB2 and 6-keto-PGF1 alpha and in decrease of platelet count were significantly relieved by intravenous infusion of yohimbine (an alpha 2-blocker), phentolamine (a nonselective alpha-blocker), and propranolol (a beta-blocker), but not by infusion of prazosin (an alpha 1-blocker). Results were compared with the control group, P less than 0.01. These results suggest that cardiac sympathetic nerves play an important role for the changes of platelet function during the early phase of myocardial ischemia and that, yohimbine and phentolamine may exert an improving effect by blocking alpha 2-receptors on the platelet membrane, and propranolol may directly contribute to stabilization of the platelet membrane.