Monif G R, Daly J W
Obstet Gynecol. 1975 Jul;46(1):80-3. doi: 10.1097/00006250-197507000-00015.
Cytologic diagnosis of adenocarcinoma metastatic to the peritoneal surface is often hampered by the difficulty of differentiating between reactive mesothelial cells and macrophages from malignant epithelial cells by light microscopy. With in vitro tissue culture technics, the fibroblastic dedifferentiation of mesothelial cells facilitates the recognition of residual neoplastic epithelial cells. Ascitic fliid from 18 patients who ultimately had histologic documentation of intraabdominal adenocarcinomatosis was studied by tissue culture technics. In 15 of the 18 patients, small islets of replicating tumor cells could be identified after the mesothelial cells had dedifferentiated into fibroblasts. With one exception the average time between collection of the specimen and recognition of adenocarcinoma was 6 days. In 14 cases, a definitive diagnosis of malignancy was made by cytologic analysis.
通过光学显微镜鉴别反应性间皮细胞、巨噬细胞与恶性上皮细胞存在困难，这常常妨碍对转移至腹膜表面的腺癌进行细胞学诊断。利用体外组织培养技术，间皮细胞的成纤维细胞去分化有助于识别残留的肿瘤上皮细胞。采用组织培养技术对18例最终经组织学证实为腹腔腺癌病的患者的腹水进行了研究。在18例患者中的15例中，间皮细胞去分化为成纤维细胞后可识别出增殖的肿瘤细胞小集落。除1例例外，标本采集至腺癌识别的平均时间为6天。14例通过细胞学分析做出了恶性肿瘤的明确诊断。
