Tanaka Y, Ueda G, Hiramatsu K, Inoue Y, Saito J, Nishino T, Sato Y, Yamasaki M, Inoue M, Kurachi K
Nihon Sanka Fujinka Gakkai Zasshi. 1983 Jul;35(7):934-8.
Atypical mesothelial cells are sometimes hard to differentiate from cancer cells in the cytology of ascitic or peritoneal washing fluid. The morphological characteristics of the cells, with or without the application of special stains, have mainly been used in differential diagnosis. In the present study, the living cytology described by Monif et al. is applied to the ascites of ovarian cancer patients. 13 cases of ovarian cancer with ascitic fluid were investigated. When 20-30ml ascitic fluid is cultured for a short term, the mesothelial cells transform to fibroblastic cells, and cancer cells form the special islets of cells. This phenomenon is judged as "Positive". The morphology of the ascitic smear and tissue culture of the tumor were also studied for comparison. Living cytology gave the same results as ascitic smear, and made it easier to differentiate mesothelial cells from cancer cells. Cancer cells in living cytology were similar to cells in tissue culture microscopically. In conclusion, living cytology seems useful especially when it is felt difficult to make a diagnosis of cancer cells on the basis of the contamination of active mesothelial cells.
在腹水或腹腔冲洗液的细胞学检查中，非典型间皮细胞有时很难与癌细胞区分开来。细胞的形态特征，无论是否应用特殊染色，主要用于鉴别诊断。在本研究中，Monif等人描述的活细胞检查法应用于卵巢癌患者的腹水。对13例伴有腹水的卵巢癌患者进行了研究。当20 - 30ml腹水短期培养时，间皮细胞转变为成纤维细胞，癌细胞形成特殊的细胞岛。此现象判断为“阳性”。还对肿瘤的腹水涂片和组织培养的形态进行了研究以作比较。活细胞检查法与腹水涂片结果相同，且更容易区分间皮细胞和癌细胞。活细胞检查法中的癌细胞在显微镜下与组织培养中的细胞相似。总之，活细胞检查法似乎很有用，尤其是当基于活跃间皮细胞的污染难以诊断癌细胞时。
