Skovsen Esben, Snyder John W, Lambert John D C, Ogilby Peter R
Department of Chemistry, and Department of Physiology, University of Aarhus, DK-8000 Arhus, Denmark.
J Phys Chem B. 2005 May 12;109(18):8570-3. doi: 10.1021/jp051163i.
In time- and spatially resolved experiments, singlet molecular oxygen, O(2)(a(1)Delta(g)), was created in a single nerve cell upon irradiation of a sensitizer incorporated in the cell nucleus using a focused laser beam. The singlet oxygen thus produced was detected by its infrared phosphorescence. Data obtained indicate that, contrary to common perception, this reactive species can be quite long-lived in a cell and, as such, can diffuse over appreciable distances including across the cell membrane into the extra-cellular environment. These results provide a new perspective for mechanistic studies of photoinduced cell death and intracellular signaling.
在时间和空间分辨实验中，通过使用聚焦激光束照射掺入细胞核中的敏化剂，在单个神经细胞中产生了单线态分子氧O₂(a¹Δg)。由此产生的单线态氧通过其红外磷光进行检测。所获得的数据表明，与通常的认知相反，这种活性物质在细胞中可以相当长寿，因此能够扩散相当远的距离，包括穿过细胞膜进入细胞外环境。这些结果为光诱导细胞死亡和细胞内信号传导的机制研究提供了新的视角。
