Breitenbach Thomas, Kuimova Marina K, Gbur Peter, Hatz Sonja, Schack Nickolass Bitsch, Pedersen Brian Wett, Lambert John D C, Poulsen Lars, Ogilby Peter R
Department of Chemistry, University of Aarhus, DK-8000, Arhus, Denmark.
Photochem Photobiol Sci. 2009 Apr;8(4):442-52. doi: 10.1039/b809049a. Epub 2008 Sep 23.
Singlet molecular oxygen, O(2)(a(1)Delta(g)), can be created in photosensitized experiments with sub-cellular spatial resolution in a single cell. This cytotoxic species can subsequently be detected by its 1270 nm phosphorescence (a(1)Delta(g)--> X(3)Sigma). Cellular responses to the creation of singlet oxygen can be monitored using viability assays. Time- and spatially-resolved optical measurements of both singlet oxygen and its precursor, the excited state sensitizer, reflect the complex and dynamic morphology of the cell. These experiments help elucidate photoinduced, oxygen-dependent events that compromise cell function and ultimately lead to cell death. In this perspective, recent work on the photosensitized production and detection of singlet oxygen in single cells is summarized, highlighting the advantages and current limitations of this unique experimental approach to study an old problem.
单线态分子氧O(2)(a(1)Delta(g))可在单细胞中以亚细胞空间分辨率的光敏实验中产生。这种细胞毒性物质随后可通过其1270纳米磷光（a(1)Delta(g)--> X(3)Sigma）进行检测。可使用活力测定法监测细胞对单线态氧产生的反应。对单线态氧及其前体（激发态敏化剂）进行时间分辨和空间分辨光学测量，可反映细胞复杂而动态的形态。这些实验有助于阐明损害细胞功能并最终导致细胞死亡的光诱导、氧依赖性事件。从这个角度出发，总结了近期关于单细胞中单线态氧的光敏产生和检测的工作，突出了这种独特实验方法在研究一个老问题时的优势和当前局限性。
