Sottini Silvia, Abbruzzetti Stefania, Viappiani Cristiano, Bettati Stefano, Ronda Luca, Mozzarelli Andrea
J Phys Chem B. 2005 Jun 16;109(23):11411-3. doi: 10.1021/jp0514224.
In this letter we report the first experimental evidence for CO rebinding to human hemoglobin from multiple geminate states. The analysis of the rebinding kinetics using a maximum entropy method allowed the identification of two distinct rebinding states within the protein matrix, which become populated under conditions of increased viscosity in a silica gel at high glycerol concentration. Our findings suggest the presence of at least two distinct docking sites for the photolyzed ligand. Assuming a minimal four-state model, we estimate the microscopic rates and the activation energies for the elementary processes.
在本信函中，我们报告了首个关于一氧化碳从多个双生态重新结合到人类血红蛋白的实验证据。使用最大熵方法对重新结合动力学进行分析，使得在蛋白质基质中识别出两种不同的重新结合状态成为可能，这两种状态在高甘油浓度下硅胶中粘度增加的条件下出现。我们的研究结果表明，对于光解配体存在至少两个不同的对接位点。假设一个最小的四态模型，我们估算了基本过程的微观速率和活化能。
