Noble D, Powell T
University Laboratory of Physiology, Oxford, U.K.
Proc Biol Sci. 1991 Nov 22;246(1316):167-72. doi: 10.1098/rspb.1991.0140.
The use of high-affinity fluorescent probes for monitoring intracellular free Ca2+ in cardiac muscle is now widespread. We have investigated the consequences of introducing intracellular buffers with the properties of Fura-2 or Indo-1 on the action potential, Ca2+ transient and contractile activity of the myocardium. Our theoretical results suggest that, at the high intracellular concentrations of these fluorescent probes used on occasion to improve the signal-to-noise ratio of the emitted fluorescence, modulation of action potential profile and attenuation of the amplitudes of the Ca2+ transient and contraction can occur, together with subtle changes in the kinetics of these events.
目前，高亲和力荧光探针用于监测心肌细胞内游离Ca2+的方法已广泛应用。我们研究了引入具有Fura-2或Indo-1特性的细胞内缓冲剂对心肌动作电位、Ca2+瞬变和收缩活动的影响。我们的理论结果表明，在有时为提高发射荧光的信噪比而使用的这些荧光探针的高细胞内浓度下，动作电位轮廓的调制、Ca2+瞬变和收缩幅度的衰减可能会发生，同时这些事件的动力学也会发生细微变化。
