呋喃-2和indo-1对自发搏动的培养心脏细胞中[钙离子]i的影响。

Effects of fura-2 and indo-1 on [Ca++]i in spontaneously beating cultured heart cells.

作者信息

Ishida H, Seguchi H, Sakata N, Ohusuzu F, Aosaki N, Nakamura H, Okino H

机构信息

Department of Physiology 2, School of Medicine, Tokai University, Kanagawa, Japan.

出版信息

Tokai J Exp Clin Med. 1991 Mar;16(1):3-9.

PMID:1780906

Abstract

Changes in contractility and intracellular free Ca([Ca++]i) were measured in spontaneously beating cultured heart cells following exposure to indo-1 acetoxymethyl ester (indo-1AM) and fura-2 acetoxymethyl ester (fura-2AM). Although a 10 microM concentration of indo-1AM slightly decreased the beating rate, 5 and 10 microM concentrations of fura-2AM produced arrest of contraction within 10 min. However, contractility returned to normal after washing. With indo-1, the diastolic and peak systolic levels of [Ca++]i were 282 +/- 23 and 1107 +/- 122 nM, respectively, and 84 +/- 8 and 582 +/- 93 nM, respectively, with fura-2. The difference between the indo-1 and fura-2 generated responses are statistically significant (p less than 0.01). These results demonstrate that indo-1 and fura-2 can affect [Ca++]i, but the influence of indo-1 is less than that of fura-2 in the heart cells.

摘要

在原代培养的自发性搏动心肌细胞中，观察indo-1乙酰氧基甲酯（indo-1AM）和fura-2乙酰氧基甲酯（fura-2AM）对心肌细胞收缩性和细胞内游离钙浓度（[Ca++]i）的影响。10μM的indo-1AM使心肌细胞搏动频率轻度降低，而5μM和10μM的fura-2AM均可使心肌细胞在10min内停止收缩，但冲洗后收缩功能可恢复。indo-1测定的心肌细胞舒张期和收缩期峰值[Ca++]i浓度分别为282±23nM和1107±122nM；fura-2测定的相应值分别为84±8nM和582±93nM，二者差异有统计学意义（P<0.01）。结果提示，indo-1和fura-2均可影响心肌细胞内[Ca++]i浓度，但fura-2的作用更为明显。