单分散磁性纳米颗粒的生物功能化及其在基于磁隧道结的传感器中作为生物分子标记物的应用。

Bio-functionalization of monodisperse magnetic nanoparticles and their use as biomolecular labels in a magnetic tunnel junction based sensor.

作者信息

Grancharov Stephanie G, Zeng Hao, Sun Shouheng, Wang Shan X, O'Brien Stephen, Murray C B, Kirtley J R, Held G A

机构信息

IBM T.J. Watson Research Center, P.O. Box 218, Yorktown Heights, New York 10598, USA.

出版信息

J Phys Chem B. 2005 Jul 7;109(26):13030-5. doi: 10.1021/jp051098c.

DOI:10.1021/jp051098c

PMID:16852617

Abstract

Monodisperse magnetic nanoparticles (NPs) could enable the ultra-sensitive magnetic detection of biological analytes. However, rendering these particles biocompatible has remained a challenge. We report the bio-functionalization and detection of 12-nm manganese ferrite NPs. We have achieved the site-specific binding of biotin-functionalized NPs onto avidin-patterned silicon oxide substrates and DNA-functionalized NPs onto complementary DNA-patterned silicon oxide substrates. Utilizing scanning SQUID microscopy, we show that these substrate-bound NPs retain their magnetic properties. Finally, we demonstrate a novel method of detecting either protein binding or DNA hybridization at room temperature using the NPs and a magnetic tunnel-junction-based biosensor situated in orthogonal magnetic fields.

摘要

单分散磁性纳米颗粒（NPs）能够实现对生物分析物的超灵敏磁性检测。然而，使这些颗粒具有生物相容性仍然是一个挑战。我们报告了12纳米锰铁氧体纳米颗粒的生物功能化及检测。我们已实现了生物素功能化纳米颗粒在抗生物素蛋白图案化氧化硅基板上的位点特异性结合，以及DNA功能化纳米颗粒在互补DNA图案化氧化硅基板上的位点特异性结合。利用扫描超导量子干涉显微镜，我们表明这些结合在基板上的纳米颗粒保留了它们的磁性。最后，我们展示了一种在室温下使用纳米颗粒以及位于正交磁场中的基于磁性隧道结的生物传感器来检测蛋白质结合或DNA杂交的新方法。