Gibasiewicz Krzysztof, Szrajner Anna, Ihalainen Janne A, Germano Marta, Dekker Jan P, van Grondelle Rienk
Department of Physics and Astronomy, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands.
J Phys Chem B. 2005 Nov 10;109(44):21180-6. doi: 10.1021/jp0530909.
Almost all photosystem I (PSI) complexes from oxygenic photosynthetic organisms contain chlorophylls that absorb at longer wavelength than that of the primary electron donor P700. We demonstrate here that the low-energy pool of chlorophylls in the PSI-LHCI complex from the green alga Chlamydomonas reinhardtii, containing five to six pigments, is significantly blue-shifted (A(max) at 700 nm at 4 K) compared to that in the PSI core preparations from several species of cyanobacteria and in PSI-LHCI particles from higher plants. This makes them almost isoenergetic with the primary donor. However, they keep the other characteristic features of "red" chlorophylls: clear spectral separation from the bulk chlorophylls, big Stokes shift revealing pronounced electron-phonon coupling, and large homogeneous and inhomogeneous broadening of approximately 170 and approximately 310 cm(-1), respectively.
几乎所有来自产氧光合生物的光系统I（PSI）复合物都含有叶绿素，这些叶绿素吸收的波长比初级电子供体P700更长。我们在此证明，来自绿藻莱茵衣藻的PSI-LHCI复合物中的低能叶绿素池含有五到六种色素，与几种蓝细菌的PSI核心制剂以及高等植物的PSI-LHCI颗粒相比，其显著蓝移（4K时在700nm处的A(max)）。这使得它们与初级供体几乎等能。然而，它们保留了“红色”叶绿素的其他特征：与大量叶绿素的清晰光谱分离、揭示明显电子-声子耦合的大斯托克斯位移，以及分别约为170和约310cm(-1)的大均匀和非均匀展宽。
