用热响应性抗蛋白质膜对固体表面进行功能化处理。

Functionalization of solid surfaces with thermoresponsive protein-resistant films.

作者信息

Teare D O H, Barwick D C, Schofield W C E, Garrod R P, Beeby A, Badyal J P S

机构信息

Department of Chemistry, Science Laboratories, Durham University, Durham DH1 3LE, England, UK.

出版信息

J Phys Chem B. 2005 Dec 1;109(47):22407-12. doi: 10.1021/jp052869f.

DOI:10.1021/jp052869f

PMID:16853918

Abstract

Pulsed plasma polymerization of N-isopropylacrylamide leads to the deposition of thermoresponsive films. The reversible (switching) behavior of these poly(N-isopropylacrylamide) surfaces has been exemplified by screening the adsorption of fibrinogen and fluorescein isothiocyanate labeled bovine serum albumin proteins by surface plasmon resonance (SPR) and fluorescence microscopy at low and elevated temperatures.

摘要

N-异丙基丙烯酰胺的脉冲等离子体聚合导致热响应性薄膜的沉积。通过在低温和高温下利用表面等离子体共振（SPR）和荧光显微镜筛选纤维蛋白原和异硫氰酸荧光素标记的牛血清白蛋白蛋白质的吸附，例证了这些聚（N-异丙基丙烯酰胺）表面的可逆（切换）行为。