Melmer G, Sherrington R, Mankoo B, Kalsi G, Curtis D, Gurling H M
Molecular Psychiatry Laboratory, Academic Department of Psychiatry, University College and Middlesex School of Medicine, London, United Kingdom.
Genomics. 1991 Nov;11(3):767-9. doi: 10.1016/0888-7543(91)90088-v.
A human neuroreceptor clone (G21), which was isolated by cross-hybridization with the human clone for the beta 2-adrenergic receptor, has recently been shown to encode the gene for the 5HT1A receptor (HTR1A) subtype. In situ hybridization to human metaphase chromosomes mapped the G21 sequence to chromosome 5 at bands 5q11.2-q13. The clone G21 recognizes a SacI RFLP with low heterozygosity (0.13). To increase the informativeness of the HTR1A locus we have isolated two new cosmid clones containing the receptor gene. No polymorphic microsatellites were present in the cosmids. However, one cosmid revealed a new TaqI RFLP that showed tight linkage to new highly polymorphic microsatellites for the loci D5S76, D5S39, and D5S6 in seven British and Icelandic reference pedigrees (maximum LOD of 13.2 with D5S76).
通过与人β2 - 肾上腺素能受体克隆进行交叉杂交分离出的一个人类神经受体克隆（G21），最近已被证明编码5HT1A受体（HTR1A）亚型的基因。对人类中期染色体进行原位杂交将G21序列定位到5号染色体的5q11.2 - q13带。克隆G21识别一种杂合性较低（0.13）的SacI限制性片段长度多态性（RFLP）。为了提高HTR1A基因座的信息含量，我们分离出了两个包含该受体基因的新黏粒克隆。黏粒中不存在多态性微卫星。然而，一个黏粒显示出一种新的TaqI RFLP，在7个英国和冰岛参考家系中，它与基因座D5S76、D5S39和D5S6的新的高度多态性微卫星紧密连锁（与D5S76的最大优势对数为13.2）。
