产朊假丝酵母ATCC 9950乙醇脱氢酶ADH1基因的分子克隆与特性分析
Molecular cloning and characterization of the alcohol dehydrogenase ADH1 gene of Candida utilis ATCC 9950.
作者信息
Park Yong-Cheol, Yun Na-Rae, San Ka-Yiu, Bennett George N
机构信息
Department of Biochemistry and Cell Biology, Rice University, 6100 Main street, Houston, TX 77005, USA.
出版信息
J Ind Microbiol Biotechnol. 2006 Dec;33(12):1032-6. doi: 10.1007/s10295-006-0154-8. Epub 2006 Jul 20.
The alcohol dehydrogenase gene (ADH1) of Candida utilis ATCC9950 was cloned and expressed in recombinant Escherichia coli. C. utilis ADH1 was obtained by PCR amplification of C. utilis genomic DNA using two degenerate primers. Amino acid sequence analysis of C. utilis ADH1 indicated that it contained a zinc-binding consensus region and a NAD(P)(+)-binding site, and lacked a mitochondrial targeting peptide. It has a 98 and 73% identity with ADH1s of C. albicans and Saccharomyces cerevisiae, respectively. Amino acid sequence analysis and enzyme characterization with various aliphatic and branched alcohols suggested that C. utilis ADH1 might be a primary alcohol dehydrogenase existing in the cytoplasm and requiring zinc ion and NAD(P)(+) for reaction.
对产朊假丝酵母ATCC9950的乙醇脱氢酶基因（ADH1）进行了克隆，并在重组大肠杆菌中进行表达。使用两条简并引物通过PCR扩增产朊假丝酵母基因组DNA获得了产朊假丝酵母ADH1。产朊假丝酵母ADH1的氨基酸序列分析表明，它含有一个锌结合共有区域和一个NAD(P)(+)结合位点，并且缺乏线粒体靶向肽。它与白色念珠菌和酿酒酵母的ADH1分别具有98%和73%的同一性。氨基酸序列分析以及用各种脂肪族和支链醇进行的酶学特性研究表明，产朊假丝酵母ADH1可能是一种存在于细胞质中的伯醇脱氢酶，反应需要锌离子和NAD(P)(+)。
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