Chávez F P, Pons T, Delgado J M, Rodríguez L
Bioindustry Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Yeast. 1998 Sep 30;14(13):1223-32. doi: 10.1002/(SICI)1097-0061(19980930)14:13<1223::AID-YEA301>3.0.CO;2-3.
The gene INV1 encoding invertase from the yeast Candida utilis has been cloned using a homologous PCR hybridization probe, amplified with two sets of degenerate primers designed considering sequence comparisons between yeast invertases. The cloned gene was sequenced and found to encode a polypeptide of 533 amino acids that contain a 26 amino-acid signal peptide and 12 potential N-glycosylation sites. The nucleotide sequences of the 5' and 3' non-coding regions were found to contain motifs probably involved in initiation, regulation and termination of gene transcription. The amino-acid sequence shows significant identity with other yeast, bacterial and plant beta-fructofuranosidases. The INV1 gene from C. utilis was able to complement functionally the suc2 mutation of S. cerevisiae.
利用同源PCR杂交探针克隆了产朊假丝酵母中编码转化酶的基因INV1，该探针是用两组简并引物扩增得到的，这两组引物是根据酵母转化酶之间的序列比较设计的。对克隆的基因进行测序后发现，它编码一个由533个氨基酸组成的多肽，该多肽含有一个26个氨基酸的信号肽和12个潜在的N-糖基化位点。发现5'和3'非编码区的核苷酸序列含有可能参与基因转录起始、调控和终止的基序。氨基酸序列与其他酵母、细菌和植物的β-呋喃果糖苷酶具有显著的同源性。产朊假丝酵母的INV1基因能够在功能上互补酿酒酵母的suc2突变。
