[越南重楼活性成分的分离与鉴定]

[Separation and identification of active constituents of Paris vietnamensis].

作者信息

Huang Yun, Cui Li-jian, Wang Qiang, Ye Wen-cai

机构信息

Department of Pharmacognosy, Hebei Medical Universty, Shijiazhuang, China.

出版信息

Yao Xue Xue Bao. 2006 Apr;41(4):361-4.

PMID:16856484

Abstract

AIM

To study the anti-tumor bioactive steroid saponins of Paris vietnamensis (Takht.).

METHODS

The constituents were isolated and purified by chromatography and their structures were identified by spectral analysis and physicochemical properties. The cytotoxic bioactivities of the constituents were determined by MTT.

RESULTS

Eleven compounds were obtained and identified as 3beta, 5alpha,6alpha-trihydroxy-7(8)-en-isospirostanol-3-O-beta-D-glucopyranosyl(1-->3) [alpha-L-rhamnopyranosyl(1-->2)]-beta-D-glucopyranoside (1), which was named as parisvietnaside A, 25 (R) diosgenin-3-O-alpha-L-arabinofuranosyl(1-->4)-beta-D-glucopyranoside (2), 25(R) diosgenin-3-O-alpha-L-rhamnopyranosyl(1-->2)-beta-D-glucopyranoside (3), 25 (R) diosgenin-3-O-alpha-L-arabinofuranosyl (1-->4) [alpha-L-rhamnopyranosyl (1-->2)]-beta-D-glucopyranoside (4), 25 (R) diosgenin-3-O-beta-D-glucopyranosyl (1-->3) [alpha-L-rhamnopyranosyl (1-->2)]-beta-D-glucopyranoside (5), 25 (R) diosgenin-3-O-alpha-L-rhamnopyranosyl (1-->4)-alpha-L-rhamnopyranosyl(1-->4) [alpha-L-rhamnopyranosyl-(1-->2)]-beta-D-glucopyranoside (6), 25 (R) pennogenin-3-O-alpha-L-arabinofuranosyl(1-->4)-beta-D-glucopyranoside (7), 25 (R) pennogenin-3-O-alpha-L-rhamnopyranosyl (1-->2)-beta-D-glucopyranoside (8), 25 (R) pennogenin-3-O-alpha-L-arabinofuranosyl (1-->4) [alpha-L-rhamnopyranosyl-(1-->2)]-beta-D-glucopyranoside (9), 25 (R) pennogenin-3-O-beta-D-glycopyranosyl (1-->3) [alpha-L-rhamnopyranosyl(1-->2)-beta-D-glucopyranoside (10) and 25 (R) pennogenin-3-O-alpha-L-rhamnopyranosyl (1-->4)-alpha-L-rhamnopyranosyl(1-->4) [alpha-L-rhamnopyranosyl-(1-->2)]-beta-D-glucopyranoside (11). Some constituents had cytotoxic bioactivities.

CONCLUSION

Compound 1 is a new spirostanol saponin, and compounds 2, 3, 6-11 were obtained from Paris vietnamensis (Takht.) for the first time. Compounds 3, 4, 6, 8 had cytotoxic bioactivities against tumor cells HepG2 and SGC-7901.

摘要

目的

研究越南重楼（Takht.）的抗肿瘤生物活性甾体皂苷。

方法

采用色谱法分离纯化其成分，并通过光谱分析和理化性质鉴定其结构。采用MTT法测定各成分的细胞毒生物活性。

结果

得到11个化合物，分别鉴定为3β,5α,6α-三羟基-7(8)-烯异螺旋甾烷醇-3-O-β-D-吡喃葡萄糖基(1→3)[α-L-鼠李吡喃糖基(1→2)]-β-D-吡喃葡萄糖苷（1），命名为越南重楼苷A；25(R)-薯蓣皂苷元-3-O-α-L-阿拉伯呋喃糖基(1→4)-β-D-吡喃葡萄糖苷（2）；25(R)-薯蓣皂苷元-3-O-α-L-鼠李吡喃糖基(1→2)-β-D-吡喃葡萄糖苷（3）；25(R)-薯蓣皂苷元-3-O-α-L-阿拉伯呋喃糖基(1→4)[α-L-鼠李吡喃糖基(1→2)]-β-D-吡喃葡萄糖苷（4）；25(R)-薯蓣皂苷元-3-O-β-D-吡喃葡萄糖基(1→3)[α-L-鼠李吡喃糖基(1→2)]-β-D-吡喃葡萄糖苷（5）；25(R)-薯蓣皂苷元-3-O-α-L-鼠李吡喃糖基(1→4)-α-L-鼠李吡喃糖基(1→4)[α-L-鼠李吡喃糖基-(1→2)]-β-D-吡喃葡萄糖苷（6）；25(R)-原薯蓣皂苷元-3-O-α-L-阿拉伯呋喃糖基(1→4)-β-D-吡喃葡萄糖苷（7）；25(R)-原薯蓣皂苷元-3-O-α-L-鼠李吡喃糖基(1→2)-β-D-吡喃葡萄糖苷（8）；25(R)-原薯蓣皂苷元-3-O-α-L-阿拉伯呋喃糖基(1→4)[α-L-鼠李吡喃糖基-(1→2)]-β-D-吡喃葡萄糖苷（9）；25(R)-原薯蓣皂苷元-3-O-β-D-吡喃葡萄糖基(1→3)[α-L-鼠李吡喃糖基(1→2)-β-D-吡喃葡萄糖苷（10）；25(R)-原薯蓣皂苷元-3-O-α-L-鼠李吡喃糖基(1→4)-α-L-鼠李吡喃糖基(1→4)[α-L-鼠李吡喃糖基-(1→2)]-β-D-吡喃葡萄糖苷（11）。部分成分具有细胞毒生物活性。