Meng Ying, Huang Shaoliang, Min Jun, Guo Zhongmin
Center for Stem Cell Research, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China.
Sci China C Life Sci. 2006 Jun;49(3):259-64. doi: 10.1007/s11427-006-0259-3.
Coagulation factors II, V, VII, VIII, IX and X are produced by hepatocytes. So factors VIII and IX deficiencies, which result in hemophilia A and B, have the potential to respond to cellular replacement therapy. Embryonic stem (ES) cells provide a unique source for therapeutic applications. Here, E14 mouse ES cells have been induced into hepatocytes in vitro. Morphology revealed that ES-derived hepatic-like cells were round or polyhedral shaped with distinct boundary of individual cells, and some arranged in trabeculae. These cells expressed endodermal- or liver-specific mRNA--transthyretin (TTR), alpha1-anti-trypsin (AAT), alpha-fetoprotein (AFP), albumin (ALB), glucose-6-phoshpatase (G6P) and tyrosine aminotransferase (TAT). Approximately (85.1 +/- 0.5)% of the ES-derived cells was stained positive green with ICG uptake. These cells were also stained magenta as a result of PAS reaction. In this paper, expression of coagulation factors VIII and IX mRNA in the ES-derived cells is documented. Therefore, ES cells might be developed as substitute donor cells for the therapy of coagulation factor deficiencies.
凝血因子II、V、VII、VIII、IX和X由肝细胞产生。因此，导致甲型和乙型血友病的VIII因子和IX因子缺乏症有可能对细胞替代疗法产生反应。胚胎干细胞（ES细胞）为治疗应用提供了独特的来源。在此，E14小鼠ES细胞已在体外被诱导分化为肝细胞。形态学显示，ES细胞来源的类肝细胞呈圆形或多面体形，单个细胞边界清晰，部分细胞排列成小梁状。这些细胞表达内胚层或肝脏特异性mRNA——转甲状腺素蛋白（TTR）、α1-抗胰蛋白酶（AAT）、甲胎蛋白（AFP）、白蛋白（ALB）、葡萄糖-6-磷酸酶（G6P）和酪氨酸转氨酶（TAT）。约（85.1±0.5）%的ES细胞来源细胞经吲哚菁绿摄取后被染成绿色阳性。这些细胞经PAS反应后也被染成品红色。本文记录了ES细胞来源细胞中凝血因子VIII和IX mRNA的表达情况。因此，ES细胞有可能被开发成为用于治疗凝血因子缺乏症的替代供体细胞。
