Hu Yaqin, Cai Jibao, Zhu Xiaolan, Gao Yun, Su Qingde
Dept. Chem., Univ. Sciand & Technol, China, Hefei 230026, China.
Ann Chim. 2006 May-Jun;96(5-6):309-16. doi: 10.1002/adic.200690032.
In this investigation, a novel two-step simultaneous distillation and solvent extraction (two-step SDE) method was developed to isolate both free and bound aroma in tobacco. Firstly, free aroma were extracted into dichloromethane by SDE for 4 h with SDE sample solution at pH 5.5. Then the SDE sample solution was adjusted to pH 2.5, bound aroma were hydrolyzed, and released as free aroma extracted by fresh dichloromethane. Following, both the free and bound aroma were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The whole isolation procedure of both free and bound aroma was carried out in one SDE apparatus and using one same sample. Especially, the procedure of isolation of bound aroma was greatly simplified. To apply and validate the proposed two-step SDE method, a traditional method were also used to isolate the bound aroma. The total bound aroma obtained by the proposed two-step SDE method and the traditional method were 119.83 mg kg(-1) and 88.9 mg kg(-1), respectively. For isolation of bound aroma, the proposed two-step SDE method was of better extraction recovery, less labourious, solvent and time consuming than the traditional method.
在本研究中，开发了一种新型的两步同时蒸馏萃取法（两步SDE法）来分离烟草中的游离香气和结合态香气。首先，在pH 5.5的SDE样品溶液中，通过SDE法将游离香气萃取到二氯甲烷中4小时。然后将SDE样品溶液调节至pH 2.5，使结合态香气水解，并以游离香气的形式被新鲜二氯甲烷萃取出来。随后，游离香气和结合态香气均通过气相色谱（GC）和气相色谱 - 质谱联用（GC - MS）进行分析。游离香气和结合态香气的整个分离过程在一个SDE装置中使用同一样品进行。特别是，结合态香气的分离过程大大简化。为了应用和验证所提出的两步SDE法，还使用传统方法来分离结合态香气。所提出的两步SDE法和传统方法获得的结合态香气总量分别为119.83 mg kg⁻¹和88.9 mg kg⁻¹。对于结合态香气的分离，所提出的两步SDE法比传统方法具有更好的萃取回收率，更省力，溶剂消耗和时间消耗更少。
