CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Talanta. 2010 Apr 15;81(1-2):650-6. doi: 10.1016/j.talanta.2009.12.054. Epub 2010 Jan 11.
An accelerated solvent extraction (ASE) procedure has been developed as a pretreatment method for chemical fingerprinting of volatile and semi-volatile components in cut tobacco. The ASE extraction conditions including temperature, operation pressure and extraction cycles were optimized to maximize extraction yield. The method was validated with repeatability, recovery and linearity. Compared with simultaneous distillation extraction (SDE), ASE provides higher extraction yields, less extraction time, lower solvent consumption and less labor time, and is more suitable for tobacco sample preparation. A typical ASE extract was analyzed by gas chromatography/time-of-flight mass spectrometry (GC-TOFMS). A total of 305 components with signal-to-noise ratio higher than 100 were tentatively identified by NIST05 and Wiley database. Finally, 36 cigarette samples from six cigarette brands were analyzed using the developed chemical fingerprinting method. Partial least squares-discriminant analysis shows good discrimination of different cigarette brands. The results indicate that ASE method can serve as high-throughput sample preparation technique for cigarette chemical fingerprint analysis.
建立了一种加速溶剂萃取（ASE）方法作为烟草中挥发性和半挥发性成分化学指纹图谱分析的预处理方法。优化了 ASE 萃取条件，包括温度、操作压力和萃取循环，以最大限度地提高萃取产率。该方法通过重复性、回收率和线性度进行了验证。与同时蒸馏萃取（SDE）相比，ASE 提供了更高的萃取产率、更短的萃取时间、更少的溶剂消耗和更少的人工时间，更适合烟草样品制备。通过气相色谱/飞行时间质谱（GC-TOFMS）对典型的 ASE 提取物进行了分析。通过 NIST05 和 Wiley 数据库，共鉴定出 305 种信噪比大于 100 的成分。最后，使用所建立的化学指纹图谱方法分析了来自六个卷烟品牌的 36 个卷烟样品。偏最小二乘判别分析表明，不同卷烟品牌具有良好的区分度。结果表明，ASE 方法可作为卷烟化学指纹分析的高通量样品制备技术。
