Van Hoeyveld Erna, Dupont Lieven, Bossuyt Xavier
Department of Laboratory Medicine, Immunology, University Hospital Leuven, Belgium.
Clin Chem. 2006 Sep;52(9):1785-93. doi: 10.1373/clinchem.2006.067546. Epub 2006 Jul 20.
We evaluated the ImmunoCAP technique for measurement of IgG specific to Aspergillus fumigatus and pigeon antigens.
We used ImmunoCAP and precipitation technique to measure concentrations of IgG to A. fumigatus or pigeon antigens in sera from 265 patients and 42 controls. We also evaluated linearity, interference, imprecision, concordance, and diagnostic accuracy of the measuring techniques.
The precipitation and ImmunoCAP technique showed moderate concordance (kappa, 0.46 for both A. fumigatus and pigeon antibodies). Specific IgG results for A. fumigatus and pigeon were linear (r = 0.98 and 0.97, respectively), with interrun reproducibility rates of 23% and 14% and maximal interference of 36.5% and 8% by lipid and 24% and 21% by hemolysis, respectively. A. fumigatus antibody concentrations were higher in patients with aspergillosis and allergic bronchopulmonary aspergillosis (ABPA) (median, 103 and 70.1 mgA/L, respectively) than in patients with other pulmonary diseases (median, 18.15-33.40 mgA/L). Antibodies to pigeon antigens were high in patients with hypersensitivity pneumonitis (median, 1024 mgA/L) but also in patients with other pulmonary diseases (median, 445 mgA/L). Antibody titers were substantially higher in patients with other pulmonary diseases and contact with pigeons (median, 1060 mgA/L) than in patients without antigen contact (median, 27.35 mgA/L) (P <0.004).
Agreement between the precipitation and ImmunoCAP technique was 86% for A. fumigatus and 70% for pigeon antigens. Highest concentrations of specific IgG to A. fumigatus were found in patients with aspergillosis and ABPA. Our results suggest that antigen contact was the most important variable affecting the presence of antibodies to pigeon antigen.
我们评估了免疫捕获技术用于检测烟曲霉和鸽抗原特异性IgG的情况。
我们使用免疫捕获和沉淀技术检测了265例患者和42例对照血清中针对烟曲霉或鸽抗原的IgG浓度。我们还评估了测量技术的线性、干扰、不精密度、一致性和诊断准确性。
沉淀法和免疫捕获技术显示出中等程度的一致性（kappa值，烟曲霉和鸽抗体均为0.46）。烟曲霉和鸽的特异性IgG结果呈线性（r分别为0.98和0.97），批间重复性率分别为23%和14%，脂质的最大干扰分别为36.5%和8%，溶血的最大干扰分别为24%和21%。曲霉病和变应性支气管肺曲霉病（ABPA）患者的烟曲霉抗体浓度（中位数分别为103和70.1mgA/L）高于其他肺部疾病患者（中位数为18.15 - 33.40mgA/L）。鸽抗原抗体在过敏性肺炎患者中较高（中位数为1024mgA/L），但在其他肺部疾病患者中也较高（中位数为445mgA/L）。与有鸽接触的其他肺部疾病患者的抗体滴度（中位数为1060mgA/L）显著高于无抗原接触的患者（中位数为27.35mgA/L）（P <0.004）。
沉淀法和免疫捕获技术对烟曲霉的一致性为86%，对鸽抗原的一致性为70%。曲霉病和ABPA患者中烟曲霉特异性IgG浓度最高。我们的结果表明，抗原接触是影响鸽抗原抗体存在的最重要变量。
