东莨菪亭碱作为测定蛋白质的荧光探针。

Scopoletine as fluorescence probe for determination of protein.

作者信息

Chen Yanjing, Yang Jinghe, Wang Zhiling, Wu Xia, Wang Fei

机构信息

Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2007 Mar;66(3):686-90. doi: 10.1016/j.saa.2006.04.012. Epub 2006 Apr 28.

DOI:10.1016/j.saa.2006.04.012

PMID:16859971

Abstract

Scopoletine (SLT), 7-hydroxy-6-methoxylcoumarin, is known to possess biological activities such as abirritating and anti-tumor, it can quench intrinsic fluorescence of bovine serum albumin (BSA) and the fluorescence intensity of itself is enhanced. So, SLT is used as fluorescence probe for quantitative determination of protein. The experiments indicate that under optimum conditions, the enhanced intensity of fluorescence is in proportion to the concentration of proteins in a wide range, and their detection limits (S/N=3) are 1.4 x 10(-8)g mL(-1) for BSA and 1.1 x 10(-8)g mL(-1) for HSA, respectively. Samples were satisfactorily determined. The interaction mechanism is also discussed.

摘要

东莨菪亭（SLT），即7-羟基-6-甲氧基香豆素，已知具有镇痛和抗肿瘤等生物活性，它能猝灭牛血清白蛋白（BSA）的固有荧光且自身荧光强度增强。因此，SLT被用作蛋白质定量测定的荧光探针。实验表明，在最佳条件下，荧光增强强度在很宽范围内与蛋白质浓度成正比，其对BSA的检测限（S/N = 3）为1.4×10⁻⁸ g mL⁻¹，对人血清白蛋白（HSA）的检测限为1.1×10⁻⁸ g mL⁻¹。样品测定结果令人满意。同时还讨论了相互作用机制。