通过新型铽（III）配合物对荧光的猝灭效应灵敏测定蛋白质。

Sensitive determination of proteins by its quenching effect on fluorescence of new terbium(III) complex.

作者信息

Leonenko Inna, Aleksandrova Daria, Yegorova Alla, Antonovich Valery, Karasyov Aleksandr

机构信息

A. V. Bogatsky Physico-Chemical Institute of National Academy of Sciences of Ukraine, Odessa 65080, Ukraine.

出版信息

Acta Pol Pharm. 2012 Jul-Aug;69(4):603-9.

PMID:22876601

Abstract

It is found that in hexamethylenetetramine (HMTA-HCl) buffer pH = 7.8, proteins can quench the fluorescence intensity of new terbium(III) complex with 6-[(1-hydroxy-3-oxo-6,7-dihydro-3H,5H-pyrido[3,2,1-ij]quinoline-2-carbonyl)-amino]-hexanoic acid (L). Based on this, a sensitive fluorimetric method for the determination of proteins is proposed. Under optimum conditions, the I0/I is in proportion to the concentration of protein in the range of 0.1-40.0 microg/mL for bovine serum albumin (BSA), 0.1-70.0 microg/mL for human serum albumin (HSA) and 0.1-40.0 microg/mL for immunoglobulin G (IgG). Their detection limits (S/N = 3) are 0.03 microg/mL. The interaction mechanism for the luminescence quenching is also studied.

摘要

研究发现，在六亚甲基四胺（HMTA-HCl）缓冲液（pH = 7.8）中，蛋白质能够猝灭新合成的铽（III）与6-[(1-羟基-3-氧代-6,7-二氢-3H,5H-吡啶并[3,2,1-ij]喹啉-2-羰基)-氨基]-己酸（L）形成的配合物的荧光强度。基于此，提出了一种灵敏的荧光分析法用于测定蛋白质。在最佳条件下，对于牛血清白蛋白（BSA），I0/I与蛋白质浓度在0.1 - 40.0 μg/mL范围内成正比；对于人血清白蛋白（HSA），在0.1 - 70.0 μg/mL范围内成正比；对于免疫球蛋白G（IgG），在0.1 - 40.0 μg/mL范围内成正比。它们的检测限（S/N = 3）为0.03 μg/mL。同时还研究了发光猝灭的相互作用机理。