Summers Kelly L, Marleau Annette M, Mahon Jeffrey L, McManus Ruth, Hramiak Irene, Singh Bhagirath
Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada.
Clin Immunol. 2006 Oct;121(1):81-9. doi: 10.1016/j.clim.2006.05.015. Epub 2006 Jul 21.
Characterization of dendritic cells (DC) in human diabetes has been restricted to monocyte-derived DC in type 1 diabetes, whose physiological relevance to endogenous DC is uncertain. Here, we provide the first report characterizing the phenotype and function of endogenous DC subsets in type 1 and type 2 diabetes. We show that DC subsets in each diabetic group exhibit normal properties concerning frequency and activation state, as determined using 4-color flow cytometry of whole blood cells. DC maturation is also intact as confirmed by their efficacious ability to stimulate T cell proliferation in an allogeneic MLR assay. Yet we found that DC are poor producers of IFN-alpha (P < 0.05) in human diabetes. IFN-alpha is a potent antiviral agent and therefore its reduced levels may interfere with T cell-mediated immune responses leading to increased susceptibility and persistence of infections in persons with diabetes.
对人类糖尿病中树突状细胞（DC）的特性描述仅限于1型糖尿病中单核细胞来源的DC，其与内源性DC的生理相关性尚不确定。在此，我们首次报告了1型和2型糖尿病中内源性DC亚群的表型和功能特征。我们发现，通过对全血细胞进行四色流式细胞术检测，每个糖尿病组中的DC亚群在频率和激活状态方面表现出正常特性。在同种异体混合淋巴细胞反应试验中，DC刺激T细胞增殖的有效能力证实了DC成熟也是完整的。然而，我们发现糖尿病患者体内的DC产生干扰素-α的能力较差（P < 0.05）。干扰素-α是一种有效的抗病毒剂，因此其水平降低可能会干扰T细胞介导的免疫反应，导致糖尿病患者感染易感性增加和感染持续存在。
