通过半定量实时聚合酶链反应诊断猫白血病病毒感染
Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction.
作者信息
Pinches Mark D G, Helps Christopher R, Gruffydd-Jones Tim J, Egan Kathy, Jarrett Oswald, Tasker Séverine
机构信息
University of Bristol, School of Clinical Veterinary Science, Langford House, Langford, Bristol BS40 5DU, UK.
出版信息
J Feline Med Surg. 2007 Feb;9(1):8-13. doi: 10.1016/j.jfms.2006.05.008. Epub 2006 Jul 24.
Abstract
In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods.
摘要
本文描述了一种用于猫白血病病毒（FeLV）前病毒的半定量实时聚合酶链反应检测法（RT-PCR）的设计与应用。在一群自然感染的猫中，将其性能与既定的FeLV诊断方法进行了评估，这些方法包括病毒分离和酶联免疫吸附测定（ELISA）。通过期望最大化方法检测发现，RT-PCR检测法具有高灵敏度（0.92）和高特异性（0.99），并且还能够检测出大量FeLV前病毒载量低但其他传统检测方法呈阴性的猫。
相似文献
1
Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction.通过半定量实时聚合酶链反应诊断猫白血病病毒感染
J Feline Med Surg. 2007 Feb;9(1):8-13. doi: 10.1016/j.jfms.2006.05.008. Epub 2006 Jul 24.
2
Detection of feline leukemia virus RNA in saliva from naturally infected cats and correlation of PCR results with those of current diagnostic methods.自然感染猫的唾液中猫白血病病毒RNA的检测以及PCR结果与当前诊断方法结果的相关性
J Clin Microbiol. 2006 Mar;44(3):916-22. doi: 10.1128/JCM.44.3.916-922.2006.
3
Shedding of feline leukemia virus RNA in saliva is a consistent feature in viremic cats.猫白血病病毒RNA在唾液中的脱落是病毒血症猫的一个持续特征。
Vet Microbiol. 2006 Jan 10;112(1):11-21. doi: 10.1016/j.vetmic.2005.10.027. Epub 2005 Nov 21.
4
Cellular segregation of feline leukemia provirus and viral RNA in leukocyte subsets of long-term experimentally infected cats.猫白血病前病毒和病毒RNA在长期实验感染猫的白细胞亚群中的细胞分离
Virus Res. 2007 Jul;127(1):9-16. doi: 10.1016/j.virusres.2007.03.008. Epub 2007 Apr 16.
5
Feline leukaemia virus proviral DNA detected by polymerase chain reaction in antigenaemic but non-viraemic ('discordant') cats.通过聚合酶链反应在抗原血症但无病毒血症（“不一致”）的猫中检测到猫白血病病毒前病毒DNA。
Arch Virol. 1997;142(2):323-32. doi: 10.1007/s007050050079.
6
Development and application of a quantitative real-time PCR assay to detect feline leukemia virus RNA.一种用于检测猫白血病病毒RNA的定量实时PCR检测方法的开发与应用。
Vet Immunol Immunopathol. 2008 May 15;123(1-2):81-9. doi: 10.1016/j.vetimm.2008.01.013. Epub 2008 Jan 19.
7
How molecular methods change our views of FeLV infection and vaccination.分子方法如何改变我们对猫白血病病毒感染和疫苗接种的看法。
Vet Immunol Immunopathol. 2008 May 15;123(1-2):119-23. doi: 10.1016/j.vetimm.2008.01.017. Epub 2008 Jan 19.
8
Long-term follow up of feline leukemia virus infection and characterization of viral RNA loads using molecular methods in tissues of cats with different infection outcomes.使用分子方法对不同感染结局的猫组织中猫白血病病毒感染的长期随访和病毒 RNA 载量的特征分析。
Virus Res. 2015 Feb 2;197:137-50. doi: 10.1016/j.virusres.2014.12.025. Epub 2014 Dec 30.
9
Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA.新型巢式重组酶聚合酶扩增（RPA）和逆转录酶-RPA 快速鉴定猫白血病病毒感染阶段
Sci Rep. 2021 Nov 11;11(1):22023. doi: 10.1038/s41598-021-01585-9.
10
Quantitation of feline leukaemia virus viral and proviral loads by TaqMan real-time polymerase chain reaction.运用TaqMan实时聚合酶链反应对猫白血病病毒的病毒载量和前病毒载量进行定量分析。
J Virol Methods. 2005 Dec;130(1-2):124-32. doi: 10.1016/j.jviromet.2005.06.017. Epub 2005 Jul 27.
引用本文的文献
1
Feline Leukemia Virus in Cats: A Novel Rapid ELISA Assay for p27 Antigen Detection.猫中的猫白血病病毒：一种用于检测p27抗原的新型快速酶联免疫吸附测定法。
Vet Med Int. 2025 May 15;2025:9914340. doi: 10.1155/vmi/9914340. eCollection 2025.
2
Epidemiology of a Hybrid Swarm: Evidence of 11 Feline Infectious Agents Circulating in a Population of Sympatric European Wildcat Hybrids and Free-Living Domestic Cats, in Scotland.杂交群体的流行病学：在苏格兰同域分布的欧洲野猫杂交种和自由放养家猫群体中11种猫科传染病原体传播的证据
Transbound Emerg Dis. 2023 Oct 5;2023:6692514. doi: 10.1155/2023/6692514. eCollection 2023.
3
Clinical findings, treatment and outcome in cats diagnosed with precursor-targeted immune-mediated anaemia in a referral hospital in the UK: 30 cases (2010-2021).英国一家转诊医院诊断为前体靶向免疫介导性贫血的猫的临床发现、治疗及结果：30例病例（2010 - 2021年）
Vet Rec Open. 2023 Aug 27;10(2):e70. doi: 10.1002/vro2.70. eCollection 2023 Dec.
4
A Retrospective Study of Viral Molecular Prevalences in Cats in Southern Italy (Campania Region).意大利南部（坎帕尼亚地区）猫科动物病毒分子流行率的回顾性研究。
Viruses. 2022 Nov 21;14(11):2583. doi: 10.3390/v14112583.
5
Feline Leukemia Virus p27 Antigen Concentration and Proviral DNA Load Are Associated with Survival in Naturally Infected Cats.猫白血病病毒 p27 抗原浓度和前病毒 DNA 载量与自然感染猫的存活相关。
Viruses. 2021 Feb 15;13(2):302. doi: 10.3390/v13020302.
6
Follow-Up of Viral Parameters in FeLV- or FIV-Naturally Infected Cats Treated Orally with Low Doses of Human Interferon Alpha.低剂量人α干扰素口服治疗自然感染猫白血病病毒或猫免疫缺陷病毒的猫的病毒参数随访
Viruses. 2019 Sep 11;11(9):845. doi: 10.3390/v11090845.
7
The Diagnosis of Feline Leukaemia Virus (FeLV) Infection in Owned and Group-Housed Rescue Cats in Australia.澳大利亚家猫和群居救助猫中猫白血病病毒（FeLV）感染的诊断。
Viruses. 2019 May 31;11(6):503. doi: 10.3390/v11060503.
8
Prevalence and risk factor analysis for feline haemoplasmas in cats from Northern Serbia, with molecular subtyping of feline immunodeficiency virus.塞尔维亚北部猫科动物猫血巴尔通体的患病率及危险因素分析，以及猫免疫缺陷病毒的分子亚型分析
JFMS Open Rep. 2018 May 22;4(1):2055116918770037. doi: 10.1177/2055116918770037. eCollection 2018 Jan-Jun.
9
Molecular Diagnosis of Felis catus Gammaherpesvirus 1 (FcaGHV1) Infection in Cats of Known Retrovirus Status with and without Lymphoma.猫γ疱疹病毒 1 型（FcaGHV1）在已知反转录病毒感染状态下猫的分子诊断，包括淋巴瘤和非淋巴瘤。
Viruses. 2018 Mar 14;10(3):128. doi: 10.3390/v10030128.
10
Rapid and sensitive insulated isothermal PCR for point-of-need feline leukaemia virus detection.用于即时需求检测猫白血病病毒的快速灵敏的绝缘等温PCR技术
J Feline Med Surg. 2018 Apr;20(4):362-369. doi: 10.1177/1098612X17712847. Epub 2017 Jun 7.
本文引用的文献
1
Use of quantitative real-time PCR to monitor the response of Chlamydophila felis infection to doxycycline treatment.运用定量实时聚合酶链反应监测猫衣原体感染对强力霉素治疗的反应。
J Clin Microbiol. 2005 Apr;43(4):1858-64. doi: 10.1128/JCM.43.4.1858-1864.2005.
2
Re-examination of feline leukemia virus: host relationships using real-time PCR.使用实时聚合酶链反应对猫白血病病毒宿主关系的重新审视。
Virology. 2005 Feb 5;332(1):272-83. doi: 10.1016/j.virol.2004.10.050.
3
Longitudinal analysis of feline leukemia virus-specific cytotoxic T lymphocytes: correlation with recovery from infection.猫白血病病毒特异性细胞毒性T淋巴细胞的纵向分析：与感染恢复的相关性
J Virol. 2002 Mar;76(5):2306-15. doi: 10.1128/jvi.76.5.2306-2315.2002.
4
"TAGS", a program for the evaluation of test accuracy in the absence of a gold standard.“TAGS”，一个用于在没有金标准的情况下评估检测准确性的程序。
Prev Vet Med. 2002 Feb 14;53(1-2):67-81. doi: 10.1016/s0167-5877(01)00272-0.
5
Feline leukaemia provirus load during the course of experimental infection and in naturally infected cats.实验性感染过程中及自然感染猫体内的猫白血病致病毒载量
J Gen Virol. 2001 Jul;82(Pt 7):1589-1596. doi: 10.1099/0022-1317-82-7-1589.
6
Feline leukaemia virus proviral DNA detected by polymerase chain reaction in antigenaemic but non-viraemic ('discordant') cats.通过聚合酶链反应在抗原血症但无病毒血症（“不一致”）的猫中检测到猫白血病病毒前病毒DNA。
Arch Virol. 1997;142(2):323-32. doi: 10.1007/s007050050079.
7
Comparative studies of the efficacy of a recombinant feline leukaemia virus vaccine.重组猫白血病病毒疫苗效力的比较研究
Vet Rec. 1996 Jan 6;138(1):7-11. doi: 10.1136/vr.138.1.7.
8
Detection of FeLV antigen.猫白血病病毒抗原的检测
Vet Rec. 1995 Jul 29;137(5):127. doi: 10.1136/vr.137.5.127-a.
9
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.CLUSTAL W：通过序列加权、位置特异性空位罚分和权重矩阵选择提高渐进多序列比对的灵敏度。
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80. doi: 10.1093/nar/22.22.4673.
Zotero 插件