通过聚合酶链反应在抗原血症但无病毒血症（“不一致”）的猫中检测到猫白血病病毒前病毒DNA。

Feline leukaemia virus proviral DNA detected by polymerase chain reaction in antigenaemic but non-viraemic ('discordant') cats.

作者信息

Miyazawa T, Jarrett O

机构信息

Department of Veterinary Pathology, University of Glasgow Veterinary School, U.K.

出版信息

Arch Virol. 1997;142(2):323-32. doi: 10.1007/s007050050079.

DOI:10.1007/s007050050079

PMID:9125046

Abstract

A nested polymerase chain reaction (PCR) was established to detect exogenous feline leukaemia virus (FeLV) proviral DNA in feline peripheral blood leukocytes (PBL). The assay detected a single copy of plasmid DNA of an infectious molecular clone of FeLV subgroup A in the sample by ethidium bromide staining in agarose gels. The utility of the nested PCR in the diagnosis of FeLV infections was compared with the detection of FeLV p27 antigen by enzyme-linked immunosorbent assay (ELISA) and virus isolation (VI). FeLV genomes were detected by PCR in all 4 samples that were positive by ELISA and VI but in none of 7 samples that were negative by the two methods. FeLV genomes were found by PCR in 13 of 39 samples from cats that were antigenaemic but from which no virus was isolated ('discordant' cats). These results demonstrated that a proportion of discordant cats harboured FeLV genome in their PBL.

摘要

建立了一种巢式聚合酶链反应（PCR）来检测猫外周血白细胞（PBL）中的外源性猫白血病病毒（FeLV）前病毒DNA。该检测方法通过琼脂糖凝胶中的溴化乙锭染色，在样本中检测到了FeLV A亚群感染性分子克隆的单拷贝质粒DNA。将巢式PCR在FeLV感染诊断中的效用与酶联免疫吸附测定（ELISA）检测FeLV p27抗原和病毒分离（VI）进行了比较。在ELISA和VI检测均为阳性的所有4个样本中，通过PCR检测到了FeLV基因组，但在两种方法检测均为阴性的7个样本中未检测到。在39只抗原血症但未分离到病毒的猫的样本中，有13个样本通过PCR检测到了FeLV基因组（“不一致”猫）。这些结果表明，一部分“不一致”猫的PBL中携带了FeLV基因组。